Summary
The effect of purified human fibroblast beta-interferon (B-IFN) and recombinant alpha-2b-interferon (A-IFN) on cell proliferation was investigated in two human prostate carcinoma cell lines, named PC-3 and DU-145. Both cell lines respond to the antiproliferative action of interferon, B-IFN being more effective than A-IFN. PC-3 is more sensitive than DU-145 cell line, showing 95% inhibition of cell proliferation at the highest concentration of B-IFN. As interferons, besides reducing cell growth, are able to modify steroid receptor content in different hormonesensitive human tumours, our results may be of some relevance as these drugs might be used to regulate both cell proliferation and hormone-sensitivity in prostate cancer.
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References
Borden EC, Balkwill FR (1984) Preclinical and clinical studies of interferons and interferon inducers in breast cancer. Cancer 53:783–788
Borden EC, Groveman DS, Nasu T, Dernikoff C, Bryan GT (1984) Antiproliferative activities of interferons against human bladder carcinoma cell lines in vitro. J Urol 132:800–803
Borden EC, Hogan IF, Voelkel JG (1982) Comparative antiproliferative activity in vitro of natural interferons alfa and beta for diploid and transformed human cells. Cancer Res 42:4948–4953
Creasey AA, Barthlomew JL, Merigan TL (1980) Role of G0-G1 arrest in the inhibition of tumour cell growth by interferon. Proc Natl Acad Sci USA 1471–1475
De Cicco F, Sica G, Benedetto MT, Ciabattoni G, Rossiello F, Nicosia A, Lupi G, Iacopino F, Mancuso S, Dell'Acqua S (1988) In vitro effects of β-interferon on steroid receptors and prostaglandin output in human endometrial adenocarcinoma. J Steroid Biochem 29:235–238
Dunnett CW (1955) A multiple comparison procedure for comparing several treatments with a control. Am Stat Assoc 50:1096–1121
Einhorn S, Strander H (1977) Is interferon tissue specific? Effect of human leukocyte and fibroblast interferons on the growth of lymphoblastoid and osteosarcoma cell lines. J Gen Virol 35:573–577
Einhorn S, Strander H (1977) Interferon therapy for neoplastic disease in man. In: Chapple PJ (ed) Human inteferon production and clinical use. Plenum, New York, pp 159–174
Epstein L, Shen JT, Abele JS, Reese CC (1980) Sensitivity of human ovarian carcinoma cells to interferon and other antitumour agents as assessed by on in vitro semi-solid agar technique. Ann NY Acad Sci 350:228–244
Fuse A, Kuwata T (1978) Inhibition of DNA synthesis and alteration of cyclic adenosine 3′, 5′ monophosphate levels in RSa cells by human leukocyte interferon. J Natl Cancer Inst 60:1227–1232
Hilfenhaus J, Damm H, Johannsen R (1977) Sensitivity of various human lymphoblastoid cells to the antiviral and anticellular activity of human leukocyte interferon. Arch Virol 54:271–277
Kaighn ME, Shankar Narayan K, Ohnuki Y, Lechner JF, Jones LW (1979) Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest Urol 17:16–23
Pfeffer LM, Murphy JS, Tamm I (1979) Interferon effects on the growth and division of human fibroblasts. Exp Cell Res 121:111–120
Shibata H, Papadimitriou JT (1981) Effects of human lymphoblastoid interferon on cultured breast cancer cells. Int J Cancer 28:447–453
Sica G, Iacopino F, Robustelli della Cuna G (1988) Recombinant alpha-2b-interferon (Intron A) modifies steroid receptor level, inhibits cell proliferation and promotes the antiproliferative activity of tamoxifen in in a human breast cancer cell line, Proceedings of the 6th Mediterranean Congress of Chemotherapy, p 99
Sica G, Natoli V, Stella C, Del Bianco S (1987) Effect of natural beta-interferon on cell proliferation and steroid receptor level in human breast cancer cells. Cancer 60:2419–2423
Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF (1978) Isolation of a human prostate carcinoma cell line (DU 145). Int J Cancer 21:274–281
Strander H, Einhorn S (1977) Effect of human leukocyte interferon on the growth of human osteosarcoma cells in tissue culture. Int J Cancer 19:468–473
Tovey M, Rocheete-Egly C, Castagna M (1979) Effect of interferon on concentrations of cyclic nucleotides in cultured cells. Proc Natl Acad Sci USA 76:3890–3893
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Supported in part by a grant of the Italian National Research Council, special project “Oncology”, contract no. 86.00520.44
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Sica, G., Fabbroni, L., Castagnetta, L. et al. Antiproliferative effect of interferons on human prostate carcinoma cell lines. Urol. Res. 17, 111–115 (1989). https://doi.org/10.1007/BF00262031
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DOI: https://doi.org/10.1007/BF00262031