Summary
The crude oil was degraded (80%) in continuous culturing and non sterile conditions by a mixed bacteria community. The fermentation process relies on a step of pre-emulsification of the substrate before it is introduced into the reactor. The emulsification indispensable for degrading crude oil is performed by the mixed bacteria community during its growth on hydrocarbons. On the other hand, the use of an ultrafiltration device allows the obtention of high cell concentrations (7.6 g·l-1) and high degradation rates.
Similar content being viewed by others
References
Atlas RM (1981) Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev 45:180–209
Bertrand JC, Rambeloarisoa E, Rontani JF, Giusti G, Mattei G (1983) Microbial degradation of crude oil in sea water in continuous culture. Biotechnol Lett 5:567–572
Colwell RR, Walker JD (1977) Ecological aspects of microbial degradation of petroleum in the marine environment. CRC Crit Rev Microbiol 5:423–445
Gutnick DL, Rosenberg E (1977) Oil tankers and pollution: a microbiological approach. Ann Rev Microbiol 31:379–396
Jannasch HW, Mateles RI (1974) Experimental bacterial ecology studied in continuous culture. In: Rose AH, Tempest DW (eds) Advances in microbial physiology, vol. 11. Academic Press, London, pp 165
Mattei G, Bertrand JC (1985) Production of biosurfactants by a mixed bacteria population grown in continuous culture on crude oil. Biotechnol Lett 4:217–222
Rambeloarisoa E, Rontani JF, Giusti G, Duvnjak Z, Bertrand JC (1984) Degradation of crude oil by a mixed population of bacteria isolated from sea-surface foams. Mar Biol 83:69–81
Sprenger B, Rehm HJ (1983) Biomass production by Candida species from n-alkanes in a film-submerged reactor in comparison with known culture methods. Eur J Appl Microbiol Biotechnol 17:265–268
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mattei, G., Rambeloarisoa, E., Giusti, G. et al. Fermentation procedure of a crude oil in continuous culture on seawater. Appl Microbiol Biotechnol 23, 302–304 (1986). https://doi.org/10.1007/BF00261933
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00261933