Summary
We have investigated the effect of pyridoxine deficiency on aromatic L-amino acid decarboxylase (AADC) using both dihydroxyphenylalanine (DOPA) and 5-hydroxytryptophan (5HTP) as substrates in the rat brain. The activity ratios of DOPA decarboxylase/5HTP decarboxylase measured under optimal substrate and cofactor concentrations were different in the cerebellum, cerebral cortex, corpus striatum and hypothalamus of the normal rat. In pyridoxine deficiency, there were no parallel decreases in DOPA and 5HTP decarboxylase activities in various brain regions. Dialysis of brain homogenates, in the presence and absence of hydroxylamine, resulted in a total or near total loss of 5HTP decarboxylase activity compared to DOPA decarboxylase activity, indicating that pyridoxal phosphate may be more tightly bound to DOPA decarboxylase than to 5HTP decarboxylase. These results, indicating that pyridoxine deficiency has differential effects on the activity of AADC, are consistent with our earlier observation of non-parallel changes in dopamine and serotonin content in various brain regions of the pyridoxine-deficient rat.
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References
Borri Voltattorni C, Minelli A, Vecchini P, Fiori A, Turano C (1979) Purification and characterization of 3,4-dihydroxyphenylalanine decarboxylase from pig kidney. Eur J Biochem 93: 181–187
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254
Cavalli-Sfoza LL, Santachiara SA, Wang L (1974) Electrophoretic study of 5-hydroxytryptophan decarboxylase from brain and liver in several species. J Neurochem 23: 629–634
Ceasar PM, Anagnoste BF, Goldstein M (1970) Purification and properties of aromatic L-amino acid decarboxylase. 160th National Meeting American Chemical Society: Abstract No. 102
Chase TN, Walters JR (1976) Pharmacologic approaches to the manipulation of GABA-mediated synaptic function in man. In: Roberts E, Chase TN, Tower DB (eds) GABA in nervous system function. Raven Press, New York, pp 497–513
Christenson JG, Dairman W, Udenfriend S (1970) Preparation and properties of a homogeneous aromatic L-amino acid decarboxylase from hog kidney. Arch Biochem Biophys 141: 356–367
Dakshinamurti K (1982) Neurobiology of pyridoxine. In: Draper HH (ed) Adv Nutr Res, Vol 4. Plenum Publishing Corp, New York, pp 143–179
Dakshinamurti K, LeBlancq WD, Herchl R, Havlicek V (1976) Nonparallel changes in brain monoamines of pyridoxine-deficient growing rats. Exp Brain Res 26: 355–366
Dakshinamurti K, Paulose CS, Thliveris JA, Vriend J (1985) Thyroid function in pyridoxine-deficient young rat. J Endocrinol 104: 339–344
Dakshinamurti K, Stephens MC (1969) Pyridoxine deficiency in the neonatal rat. J Neurochem 16: 1515–1522
Finberg JPM, Youdim MBH (1983) Monoamine oxidases. In: Lajtha A (ed) Handbook of neurochemistry, 2nd edn, Vol 4. Enzymes in the nervous system. Plenum Press, New York, pp 293–313
Glowinski J, Iversen LL (1966) Regional studies of catecholamines in the rat brain-I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain. J Neurochem 13: 655–669
Goldstein M (1972) Enzymes involved in the catalysis of catecholamine biosynthesis. In: Marks N, Rodnight R (eds) Res methods neurochem, Vol 1. Plenum Press, New York, pp 317–340
Haber B, Kuriyama K, Roberts E (1970) An anion stimulated L-glutamic acid decarboxylase in non-neural tissues. Biochem Pharmacol 19: 1119–1136
Kuhn DM, Lovenberg W (1983) Hydroxylases. In: Lajtha A (ed) Handbook of neurochemistry, 2nd edn, Vol 4. Enzymes in the nervous system. Plenum Press, New York, pp 133–150
Kuntzman R, Shore PA, Bogdanski D, Brodie BB (1961) Microanalytical procedures for fluorometric assay of brain DOPA-5HTP decarboxylase, norepinephrine and serotonin, and a detailed mapping of decarboxylase activity in brain. J Neurochem 6: 226–232
Laduron P, Belpaire F (1968) A rapid assay and partial purification of DOPA decarboxylase. Anal Biochem 26: 210–218
Lancaster GA, Sourkes TL (1972) Purification and properties of hog-kidney 3,4-dihydroxyphenylalanine decarboxylase. Can J Biochem 50: 791–797
Lipson MH, Kraus JP, Solomon LR, Rosenberg LE (1980) Depletion of cultured human fibroblasts of pyridoxal 5′phosphate: effect on activities of aspartate aminotransferase, alanine aminotransferase, and cystathionine β-synthase. Arch Biochem Biophys 204: 486–493
Lovenberg W, Victor SJ (1974) Regulation of tryptophan and tyrosine hydroxylase. Life Sci 14: 2337–2353
Lovenberg W, Weissbach H, Udenfriend S (1962) Aromatic L-amino acid decarboxylase. J Biol Chem 237: 89–93
Montjar M, Axelrod AE, Trakatellis AC (1965) Effect of pyridoxine deficiency upon polysomes and messenger RNA or rat tissues. J Nutr 85: 45–51
Murali DK, Radhakrishnan AN (1970) Purification and properties of 5-hydroxytryptophan decarboxylase from monkey small intestine. Ind J Biochem 7: 13–18
Oka K, Ashiba G, Sugimoto T, Matsuura S, Nagatsu T (1982) Kinetic properties of tyrosine hydroxylase purified from bovine adrenal medulla and bovine caudate nucleus. Biochem Biophys Acta 706: 188–196
Paulose CS, Dakshinamurti K (1985) Effect of pyridoxine deficiency in young rats on high affinity serotonin and dopamine receptors. J Neurosci Res (in press)
Rahman MK, Nagatsu T (1982) Demonstration of aromatic Lamino acid decarboxylase in human brain with L-DOPA and L-5-hydroxytryptophan as substrates by high-performance liquid chromatography with electrochemical detection. Neurochem Int 4: 1–6
Rahman MK, Nagatsu T, Kato T (1981a) Determination of aromatic L-amino acid decarboxylase in serum of various animals by high-performance liquid chromatography with electrochemical detection. Life Sci 28: 485–492
Rahman MK, Nagatsu T, Kato T (1981b) Aromatic L-amino acid decarboxylase activity in central and peripheral tissues and serum of rats with L-DOPA and L-5-hydroxytryptophan as substrates. Biochem Pharmacol 30: 645–649
Rahman MK, Nagatsu T, Sakurai T, Hori S, Abe M, Matsuda M (1982) Effect of pyridoxal phosphate deficiency on aromatic L-amino acid decarboxylase activity with L-DOPA and L-5-hydroxytryptophan as substrates in rats. Jpn J Pharmacol 32: 803–811
Schott HF, Clark WG (1952) DOPA decarboxylase inhibition through the interaction of coenzyme and substrate. J Biol Chem 196: 449–462
Sims KL, Davis GA, Bloom FE (1973) Activities of 3,4-dihydroxyL-phenylalanine and 5-hydroxy-L-tryptophan decarboxylases in rat brain: assay characteristics and distribution. J Neurochem 20: 449–464
Srinivasan K, Awapara J (1978) Substrate specificity and other properties of DOPA decarboxylase from guinea pig kidneys. Biochim Biophys Acta 526: 597–604
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Siow, Y.L., Dakshinamurti, K. Effect of pyridoxine deficiency on aromatic L-amino acid decarboxylase in adult rat brain. Exp Brain Res 59, 575–581 (1985). https://doi.org/10.1007/BF00261349
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DOI: https://doi.org/10.1007/BF00261349