Abstract
Using in vivo and in vitro experimental models, the uptake and excretion of 67Ga-citrate in tumor cells and normal cells were studied. The timelapse accumulation of 67Ga in the tumor of rats bearing Yoshida sarcoma reached its peak 24 h after the administration of 67Ga and gradually decreased thereafter. However, the excretion of 67Ga from the tumor was less than that from normal lung. For culture cells in vitro, the uptake of 67Ga increased with lapse of contact time between 67Ga and the cells, but there was no distinct difference between the results for tumor cells and normal skin fibroblasts. The excretion of 67Ga from the cells tended to decrease with prolongation of the contact time, the excretion from tumor cells being only about 10% after a contact time of 24 h. This indicated a significant delay in excretion in comparison with that of normal skin fibroblasts. This delay in the excretion of 67Ga may be an important factor in the tumor accumulation of 67Ga.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Awano, T., Matsuzawa, T.: Accumulation and biological effects of gallium in malignant cell lines in vitro (in Japanese). Jap. J. Nucl. Med. 14, 73–81 (1977)
Edwards, C.L., Hayes, R.L.: Tumor scanning with 67Ga citrate. J. Nucl. Med. 10, 103–105 (1969)
Glickson, J.D., Ryel, R.B., Bordenca, M.M., Kim, K.H., Gams, R.A.: In vitro binding of 67Ga to L 1210 cells. Cancer Res. 33, 2706–2713 (1973)
Glickson, J.D., Webb, J., Gams, R.A.: Effects of buffers and pH on in vitro binding of 67Ga by L 1210 leukemic cells. Cancer Res. 34, 2957–2960 (1974)
Gullino, P.M.: The internal milieu of tumors. Prog. Exp. Tumor Res. 8, 1–25 (1966)
Hayes, R.L.: Factors affecting uptake of radioactive agents by tumor and other tissues. In: Tumor Localization with Radioactive Agents. International Atomic Energy Agency, Vienna, pp. 29–45 (1976)
Hayes, R.L.: 67Gallium as a tumor scanning agent. ORAU 110, 1969, pp. 81–85. Research Report. Oak Ridge: Medical Division of Oak Ridge Associated Universities, 1969
Hisada, K., Tonami, N., Hiraki, T., Ando, A.: Tumor scanning with 169Yb-eitrate. J. Nucl. Med. 14, 772–773 (1973)
Hunter W.W., Riccobono, X.J.: Clinical evaluation of 111In for localization of recognized neoplastic disease. J. Nucl. Med. 11, 328–329 (1970)
Ito, Y., Okuyama, S., Sato, K., Takahashi, K., Sato, T., Kanno, I.: 67Ga tumor scanning and its mechanisms studied in rabbits. Radiology 100, 357–362 (1971)
Ito, Y., Okuyama, S., Awano, T., Kanno, I.: Diagnostic evaluation of 67Ga scanning of lung cancer and other diseases. Radiology 101, 355–362 (1971)
Ito, Y., Muranaka, A., Harada, T., Matsudo, A., Yokobayashi, T., Terashima, H.: Experimental study on tumor affinity of 201Tl-chloride. Eur. J. Nucl. Med. 3, 81–86 (1978)
Ito, Y., Okuyama, S., takahashi, K., Sato, T., Nakamura, K., Ichikawa, T., Muranaka, A.: Role of inflammation in tumorous accretion of radiopharmaceuticals: Experimental studies with 67Ga, 169Yb, 111In, 99m-Tc-Pertechnetate, 99mTc-Bleomycin, 57Co-Bleomycin and, 198Au-Colloid. Radioisotopes 24, 410–415 (1975)
Kimoto, T., Ueki, A., Nishitani, K.: A human malignant cell line established from ascites of patient with embryonal carcinoma of ovarium. Acta Path. Jap. 25, 89–98 (1975)
Langhammer, H., Glaubitt, G., Grebe, S.F., Hampe, J.F., Haubold, U., Hör, G., Kaul, A., Koeppe, P., Koppenhagen, J., Roedler, H.D., Schoot, J.B.: 67Ga for tumor scanning. J. Nucl. Med. 13, 25–30 (1972)
Mori, T., Hamamoto, K.: Fundamental and clinical studies on 99mTc labeled Bleomycin, a new tumor scanning agent (in Japanese). Jap. J. Nucl. Med. 9, 353 (1972)
Nouel, J-P., Renault, H., Robert, J., Jeanne, C., Wicent, L.: La bléomycine marquée au Co 57. La Nouvelle Presse Medicale 1, 95–98 (1972)
Orii, H.: Tumor scanning with gallium (67Ga) and its mechanism studies in rats. Strahlentherapie 144, 192–200 (1972)
Paterson, A.H.G., Yoxall, A., Smith, I., Taylor, D.M., McCready, V.R.: The concentration of 67Ga and 45Ca in the lactating mammary gland and its relevance to the tumor uptake of 67Ga citrate. Cancer Res. 36, 452–457 (1976)
Salvatore, M., Carrati, L., Bazzicalupo, L., Mansi, L.: The use of Tl-201 thallium chloride as a positive tumor indicator. Eur. J. Nucl. Med. 1, 95 (1976)
Swartzendruber, D.C., Nelson, B. and Hayes, R.L.: Gallium-67 localization in lysosomal-like granules of leukemic and non-leukemic murine tissues. J. Natl. Cancer Inst. 46, 941–952 (1972)
Tsan, M.F., Chen, W.Y., Scheffel, U., Wagner, H.N.: Studies on gallium accumulation in inflammatory lesions: 1. Gallium uptake by human polymorphonuclear leukocytes. J. Nucl. Med. 19, 36–43 (1978)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Muranaka, A., Ito, Y., Hashimoto, M. et al. Uptake and excretion of 67Ga-citrate in malignant tumors and normal cells. Eur. J. Nucl. Med. 5, 31–37 (1980). https://doi.org/10.1007/BF00261204
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00261204