Summary
Three triploid (2n=3x=36) blueberry hybrids were obtained by hand-pollinating approximately 7,000 flowers of tetraploid highbush blueberry cultivars (based on Vaccinium corymbosum L.) with pollen from the diploid species V. elliottii Chapm. Meiotic analysis of these triploids revealed trivalents, bivalents and univalents in all metaphase I cells, with lagging chromosomes evident at anaphase I. Pollen of the three triploids was mostly aborted and did not stain with acetocarmine. However, the three triploids did produce from 0.9%–1.3% giant pollen grains that stained with acetocarmine and were present as monads, dyads or triads, rather than the normal tetrads. Pollination of 10,853 flowers of hexaploid V. ashei Reade cultivars with pollen from the triploids produced 266 berries, which averaged fewer than two fully-developed seeds per berry. One triploid clone showed partial female fertility when crossed to hexaploids, self-pollinated, or intercrossed with other triploids. Ploidy levels of the resulting hybrids were determined.
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References
Ahokas H (1971) Notes on polyploidy and hybridity in Vaccinium species. Ann Bot Fenn 8:254–256
Ballington JR, Galletta GJ (1978) Comparative crossability of 4 diploid Vaccinium species. J Am Soc Hortic Sci 103:554–560
Camp WH (1945) The north American blueberries with notes on other groups of Vacciniaceae. Brittonia 5:203–275
Cockerham LE, Galletta GJ (1976) A survey of pollen characteristics in certain Vaccinium species. J Am Soc Hortic Sci 101:671–676
Darrow GM, Camp WH (1945) Vaccinium hybrids and the development of new horticultural material. Bull Torrey Bot Club 72:1–21
Darrow GM, Morrow EB, Scott DH (1952) An evaluation of interspecific blueberry crosses. Proc Am Soc Hortic Sci 59:277–282
El-Agamy SZA, Sherman WB, Lyrene PM (1981) Fruit set and seed number from self-and cross-pollinated high bush (4x) and rabbiteye (6x) blueberries. J Am Soc Hortic Sci 106: 443–445
Galletta GJ (1975) Blueberries and cranberries. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue University Press, West Lafayette, pp 154–196
Goldy RG (1983) Heteroploid gene transfers in Vaccinium section Cyanococcus. PhD thesis. University of Florida, Gainesville/FL, USA
Jelenkovic G, Draper AD (1973) Breeding value of pentaploid interspecific hybrids of Vaccinium. Jugosl Vocarstvo 7 (25/26):237–244
Jelenkovic G, Hough LF (1970) Chromosome associations in the first meiotic division in three tetraploid clones of Vaccinium corymbosum L. Can J Genet Cytol 12:316–324
Kloet SP Van der (1977) Potential and actual gene exchange among three sympatric species of Vaccinium Section Cyanococcus in Highlands County, Florida. Can J Bot 55:2668–2672
Kloet SP Van der (1983) The taxonomy of Vaccinium cyanococcus: a summation. Can J Bot 61:256–266
Lyrene PM, Sherman WB (1980) Horticultural characteristics of native Vaccinium darrowi, V. elliottii, V. fuscatum, and V. myrsinites in Alachua County, Florida. J Am Soc Hortic Sci 105:393–396
Lyrene PM, Sherman WB (1983) Mitotic instability and 2n gamete production in Vaccinium corymbosum x V. elliottii hybrids. J Am Soc Hortic Sci 108:339–342
Megalos BS, Ballington JR (1987) Pollen viability in five Southeastern United States diploid species of Vaccinium. J Am Soc Hortic Sci 112:1009–1012
Moore JN, Scott DH, Dermen H (1964) Development of a decaploid blueberry by colchicine treatment. Proc Am Soc Hortic Sci 84:274–279
Munoz CE, Lyrene PM (1985) In vitro attempts to overcome the cross-incompatibility between Vaccinium corymbosum L. and V. elliottii Chapm. Theor Appl Genet 69:591–596
Sharpe RH, Darrow GM (1959) Breeding blueberries for the Florida climate. Proc Fla State Hortic Soc 72:308–311
Sharpe RH, Sherman WB (1971) Breeding blueberries for low chilling requirement. HortScience 6:145–147
Vorsa N, Jelenkovic G, Draper AD, Welker WV (1987) Fertility of 4xx5x and 5xx4x progenies derived from Vaccinium ashei/corymbosum pentaploid hybrids. J Am Soc Hortic Sci 112:993–997
Woodell SRJ, Valentine DH (1961) Studies in British Primulas IX. Seed incompatibility in diploid-autotetraploid crosses. New Phytol 60:282–295
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Communicated by P. L. Pfahler
Florida Agricultural Experiment Station Journal Series No. 8672
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Dweikat, I.M., Lyrene, P.M. Production and viability of unreduced gametes in triploid interspecific blueberry hybrids. Theoret. Appl. Genetics 76, 555–559 (1988). https://doi.org/10.1007/BF00260907
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DOI: https://doi.org/10.1007/BF00260907