Summary
We previously described the isolation and the nucleotide sequence of a nuclear gene from sorghum (NMDHI; 4.6 kb) encoding the NADP-malate dehydrogenase. Further analysis led us to identify a second homologous gene (NMDH II; 4.8 kb) located within the same 12.3 kb genomic clone (λLM17); these two genes are tandemly organized, in direct orientation. This second gene was entirely sequenced and comparison with the first gene showed that the positions on the 14 exons and 13 introns are conserved in both genes. The analysis of the genomic organization and copy number in the Sorghum vulgare genome revealed that there are no additional homologues and there is only one copy each of NMDH I and NMDH II. The isolation of two different cDNA clones in a previous work suggested that both genes were probably expressed. Analysis of specific mRNA accumulation during the greening process using synthetic oligonucleotide probes showed that the NMDH I gene is induced in the presence of light while the NMDH II gene seems to be constitutively expressed at low level.
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Abbreviations
- Cab:
-
chlorophyll a/b binding protein
- CTAB:
-
N-cetyl-N,N,N-trimethyl-ammonium bromide
- NADP-MDH:
-
NADP-dependent dehydrogenase
- RbcS:
-
ribulose-1,5-bisphosphate carboxylase small subunit
- SSC:
-
0.15 M NaCl, 0.015 M sodium citrate pH 7.6
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Communicated by A. Kondorosi
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Luchetta, P., Crétin, C. & Gadal, P. Organization and expression of the two homologous genes encoding the NADP-malate dehydrogenase in Sorghum vulgare leaves. Molec. Gen. Genet. 228, 473–481 (1991). https://doi.org/10.1007/BF00260642
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DOI: https://doi.org/10.1007/BF00260642