Summary
We have transformed Schizosaccharomyces pombe with the β-glucuronidase (GUS) gene from Escherichia coli under the control of the plant cauliflower mosaic virus (CaMV) 35S promoter element. Efficient expression of GUS enzyme was observed. Moreover, transcription initiated at a unique site identical to that used in plant cells.
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References
Coraggio I, Compagno C, Martegani E, Ranzi BM, Sala E, Alberghina L, Viotti A (1986) Transcription and expression of zein sequences in yeast under natural plant or yeast promoters. EMBO J 5:459–465
Cramer JH, Lea K, Slightom JL (1985) Expression of phaseolin cDNA genes in yeast under control of natural plant DNA sequences. Proc Natl Acad Sci USA 82:334–338
Edens L, Bom I, Ledeboer AM, Maat J, Toonen MY, Visser C, Vampa CT (1984) Synthesis and processing of the plant protein thaumatin in yeast. Cell 37:629–633
Franck A, Guilley H, Jonard G, Richards K, Hirth L (1980) Nucleotide sequence of Cauliflower mosaic virus DNA. Cell 21:285–294
Gatermann KB, Rosenberg GH, Kaufer NF (1988) Double-stranded sequencing, using mini-prep plasmids, in eleven hours. Biotechnology 6:952–953
Gatermann KB, Hoffmann A, Rosenberg GH, Käufer NF (1989) Introduction of functional artifical introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe. Mol Cell Biol 19:1526–1535
Grimm C, Kohli J, Murray J, Maundrell K (1988) Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet 215:81–86
Guilley H, Dudley RK, Jonard G, Balazs E, Richards KE (1982) Transcription of Cauliflower mosaic virus DNA: Detection of promoter sequences, and characterization of transcripts. Cell 30:763–773
Hawkins JD (1988) A survey on intron and exon lengths. Nucleic Acids Res 16:9893–9908
Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405
Jefferson RA, Burgess SM, Hirsh D (1986) β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci USA 83:8447–8451
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
Jensen EO, Marcker KA, Villadsen IS (1986) Heme regulates the expression in Saccharomyces cerevisiae of chimaeric genes containing 5′-flanking soybean leghemoglobin sequences. EMBO 15:843–847
Langridge P, Eibel H, Brown JWS, Feix G (1984) Transcription from maize storage protein gene promoters in yeast. EMBO J 3:2467–2471
Lee MG, Nurse P (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature 327:31–35
Neill JD, Litts JC, Anderson OD, Greene FC, Stiles JI (1987) Expression of a wheat α-gliadin gene in Saccharomyces cerevisiae. Gene 55:303–317
Odell JT, Nagy F, Chua N-H (1985) Identification of DNA sequences required for activity of the Cauliflower mosaic virus 35S promoter. Nature 313:810–812
Odell JT, Knowlton S, Lin W, Mauvais CJ (1988) Properties of an isolated transcription stimulating sequence derived from the Cauliflower mosaic virus 35S promoter. Plant Mol Biol 10:263–272
Ow DW, Jacobs JD, Howell SH (1987) Functional regions of the Cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity. Proc Natl Acad Sci USA 84:4870–4874
Rothstein SJ, Lazarus CM, Smith WE, Baulcombe DC, Gatenby AA (1984) Secretion of a wheat α-amylase expressed in yeast. Nature 308:662–665
Russell PR (1983) Evolutionary divergence of the mRNA transcription initiation mechanism in yeast. Nature 301:167–169
Russell P, Nurse P (1986) Schizosaccharomyces pombe and Saccharomyces cerevisiae: a look at yeasts divided. Cell 45:781–782
Wright A, Maundrell K, Heyer W-D, Beach D, Nurse P (1986) Vectors for the construction of gene banks and the integration of cloned genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae. Plasmid 15:156–158
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Communicated by C.P. Hollenberg
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Pobjecky, N., Rosenberg, G.H., Dinter-Gottlieb, G. et al. Expression of the β-glucuronidase gene under the control of the CaMV 35S promoter in Schizosaccharomyces pombe . Molec. Gen. Genet. 220, 314–316 (1990). https://doi.org/10.1007/BF00260500
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DOI: https://doi.org/10.1007/BF00260500