Summary
Three linear DNA plasmids were found in isolate RI-64 of anastomosis group 4 (AG-4) of Rhizoctonia solani. These plasmids, designated pRS64-1, -2, and -3, possessed the same size of 2.7 kb. Restriction mapping and Southern hybridization analysis of pRS64-1, -2, and -3 revealed the presence of homologous regions at both termini. The plasmid DNAs were resistant to both 3′-exonuclease and 5′-exonuclease even after treatment with proteinase K or alkali. The length of both terminal fragments that were generated by restriction endonuclease digestion was doubled under the denaturation condition, indicating that the linear plasmid DNAs have hairpin loops at both termini. Southern blotting analysis of total DNA showed the presence of two types of dimeric forms of pRS64 DNA. One is a head-to-head dimer and the other is a tail-to-tail dimer. The role of these unique DNA structures in replication of the plasmids is discussed.
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Anagnostakis SL (1982) Biological control of chestnut blight. Science 215:466–471
Barbour AG, Garon CF (1987) Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends. Science 237:409–411
Baroudy BM, Venkatesan S, Moss B (1982) Incompletely basepaired flip-flop terminal loops link the two DNA strands of the vaccinia virus genome into one uninterrupted polynucleotide chain. Cell 28:315–324
Baroudy BM, Venkatesan S, Moss B (1983) Structure and replication of vaccinia virus telomeres. Cold Spring Harbor Symp Quant Biol 47:723–729
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Blackburn EH, Gall JG (1978) A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol 120:33–53
Düvell A, H-Stutzke H, Oeser B, R-Backwinkel P, Tudzynski P (1988) Structural and functional analysis of mitochondrial plasmids in Claviceps purpurea. Mol Gen Genet 214:128–134
Esser K, Kempken F (1986) Structure and function of linear extrachromosomal DNA in eukaryotes. Proc Biochem 21:69–75
Forte MA, Fangman WL (1979) Yeast chromosomal DNA molecules have strands which are cross-linked at their termini. Chromosoma 72:131–150
Geshelin P, Berns KI (1974) Characterization and localization of the naturally occurring cross-links in vaccinia virus DNA. J Mol Biol 88:785–796
Giasson L, Lalonde M (1987) Analysis of a linear plasmid isolated from the pathogenic fungus Ceratocystis fimbriata Ell. & Halst. Curr Genet 11:331–334
Gunge N (1983) Yeast DNA plasmids. Annu Rev Microbiol 37:253–276
Gunge N, Tamaru A, Ozawa F, Sakaguchi K (1981) Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid associated killer character. J Bacteriol 145:382–390
Hashiba T (1988) Role of linear DNA plasmids in plant pathogenic fungi. 5th International Congress of Plant Pathology (abst.) p12
Hashiba T, Homma Y, Hyakumachi M, Matsuda I (1984) Isolation of a DNA plasmid in the fungus Rhizoctonia solani. J Gen Microbiol 130:2067–2070
Hauswirth WW, Berns KI (1979) Adeno-associated virus DNA replication: nonunit-length molecules. Virology 93:57–68
Hayakawa T, Tanaka T, Sakaguchi K, Otake N, Yonehara H (1979) A linear plasmid-like DNA in Streptomyces sp. producing lankacidin group antibiotics. J Gen Appl Microbiol 25:255–260
Hirochika H, Sakaguchi K (1982) Analysis of linear plasmids isolated from Streptomyces: Association of protein with the ends of the plasmid DNA. Plasmid 7:59–65
Hirochika H, Nakamura K, Sakaguchi K (1984) A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs. EMBO J 3:761–766
Hirt B (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26:365–369
Honeyman AL, Currier TC (1986) Isolation and characterization of linear DNA elements from the mitochondria of Gaeumannomyces graminis. Appl Environ Microbiol 52:924–929
Kemble RJ, Bedbrook JR (1980) Low molecular weight circular and linear DNA in mitochondria from normal and male-sterile Zea mays cytoplasm. Nature 284:565–566
Kikuchi Y, Hirai K, Hishinuma F (1984) The yeast linear DNA killer plasmids pGKL1 and pGKL2, possess terminally attached proteins. Nucleic Acids Res 12:5685–5692
Kikuchi Y, Hirai K, Gunge N, Hishinuma F (1985) Hairpin plasmid — a novel linear DNA of perfect hairpin structure. EMBO J 4:1881–1886
Kistler HC, Leong SA (1986) Linear plasmid like DNA in the plant pathogenic fungus Fusarium oxysporum f. sp. conglutinans. J Bacteriol 167:587–593
Kornberg A (1980) DNA replication. WH Freeman, San Francisco
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Miyasaka A, Hashiba T, Ehara Y (1988) Detection and properties of plasmid like DNA in isolates from 6 anastomosis groups of Rhizoctonia solani. 5th International Congress of Plant Pathology (abst.) p 197
Miyashita S, Hirochika H, Ikeda J, Hashiba T (1988) Linear plasmids from Rhizoctonia solani with unique terminal structures. 5th International Congress of Plant Pathology (abst.) p 197
Prichard AE, Cummings DJ (1981) Replication of linear mitochondrial DNA from Paramecium: Sequence and structure of the initiation-end crosslink. Proc Natl Acad Sci USA 78:7341–7345
Pring DR, Levings CS III, Hu WWL, Timothy DH (1977) Unique DNA associated with mitochondria in the “S”-type cytoplasm of male-sterile maize. Proc Natl Acad Sci USA 74:2904–2908
Salas M (1983) A new mechanism for the initiation of replication of ϕ 29 and adenovirus DNA: priming by the terminal protein. Curr Top Microbiol Immunol 109:89–106
Samac DA, Leong SA (1988) Two linear plasmids in mitochondria of Fusarium solani f. sp. cucurbitae. Plasmid 19:57–67
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Sato M, Nishiyama K, Shirata A (1983) Involvement of plasmid DNA in the productivity of coronatine by Pseudomonas syringae pv. atropurpurea. Ann Phytopathol See Jpn 48:27–33
Stillman BW (1983) The replication of adenovirus DNA with purified proteins. Cell 35:7–9
Straus SE, Sebring ED, Rose JA (1976) Concatemers of alternating plus and minus strands are intermediates in adenovirus-associated virus DNA synthesis. Proc Natl Acad Sci USA 73:742–746
Tattersall P, Ward DC (1976) Rolling hairpin model for replication of parvovirus and linear chromosomal DNA. Nature 263:106–109
Tudzynski P, Düvell A, Esser K (1983) Extrachromosomal genetics of Claviceps purpurea. I. Mitochondrial DNA and mitochondrial plasmids. Curr Genet 7:145–150
Van Larebeke N, Engler G, Holsters M, Van den Elsacker S, Zaenen I, Schilperoort RA, Schell J (1974) Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature 252:169–170
Watson B, Currier TC, Gordon MP, Chilton M-D, Nester EW (1975) Plasmid required for virulence of Agrobacterium tumefaciens. J Bacteriol 123:255–264
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Miyashita, Si., Hirochika, H., Ikeda, JE. et al. Linear plasmid DNAs of the plant pathogenic fungus Rhizoctonia solani with unique terminal structures. Molec. Gen. Genet. 220, 165–171 (1990). https://doi.org/10.1007/BF00260476
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DOI: https://doi.org/10.1007/BF00260476