Summary
Mature macrogametes of Eimeria bovis in the mucosa of the colon of calves killed 17 days after inoculation had a characteristic fine structure. Numerous osmiophilic wall-forming bodies of type I were located peripherally in the macrogamete, whereas the wall-forming bodies of type II were relatively large and located more centrally. Numerous polysaccharide bodies and lipid inclusions were also present. The parasitophorous vacuole, when present, was narrow and had electron-pale contents. The host cell cytoplasm was vacuolated; in some specimens the host cell appeared to have disintegrated. A microgamete was observed within a vacuole in the cytoplasm of a macrogamete from a calf killed 19 days after inoculation. This microgamete had two flagella and a homogeneous osmiophilic nucleus. The wall-forming bodies of type I and type II were altered in this macrogamete as compared with those of mature macrogametes, indicating that oocyst wall formation had begun. Many microgametes were observed in the cytoplasm of the host cell as well as in the parasitophorous vacuole of the macrogamete.
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Cheissin, E. M.: Electron microscope study of microgametogenesis in two species of coccidia from rabbit (E. magna and E. intestinalis). Acta Protozool. 3, 215–224 (1965).
Colley, F. C.: Fine structure of microgametocytes and macrogametes of Eimeria nieschulzi. J. Protozool. 14 (4), 663–674 (1967).
Hammond, D. M., Scholtyseck, E., Miner, M. L.: The fine structure of microgametocytes of E. perforans, E. stiedae, E. bovis, E. auburnensis. J. Parasit. 53, (2) 235–247 (1967).
Scholtyseck, E.: Die Mikrogametenenentwicklung von E. perforans. Z. Zellforsch. 66, 625–642 (1965).
— Gönnert, R., Haberkorn, A.: Die Feinstruktur der Makrogameten des Hühnercoccids Eimeria tenella. Z. Parasitenk. 33, 31–43 (1969).
— Hammond, D. M., Ernst, J. V.: Fine structure of the macrogametes of E. perforans, E. stiedae, E. bovis, E. auburnensis. J. Parasit. 52 (5), 975–987 (1966).
— Mehlhorn, H.: Ultrastructural study of characteristic organelles (paired organelles, micronemes, micropores) of Sporozoa and related organisms. Z. Parasitenk. 34, 97–127 (1970).
— Rommel, A., Heller, G.: Licht- und elektronenmikroskopische Untersuchungen zur Bildung der Oocystenhülle bei Eimerien (E. perforans, E. stiedae, E. tenella). Z. Parasitenk. 31, 289–298 (1969).
— Schäfer, D.: Über schlauchförmige Ausstülpungen an der Zellmembran der Makrogametocyten von E. perforans. Z. Zellforsch. 61, 214–219 (1963).
— Voigt, W.-H.: Die Bildung der Oocystenhülle bei E. perforans (Sporozoa). Z. Zellforsch. 62, 279–292 (1963).
Sjöstrand, F. S.: Electron microscopy of cells and tissues. In: Oster and Pollister (eds.), Physical techniques in biological research, vol. 3, p. 241–298. New York: Academic Press 1956.
Snigirevskaya, E. S.: The occurrence of micropore in schizonts, microgametocytes and macrogametes of E. intestinalis. Acta Protozool. 5 (25), 381–396 (1968).
— Electron microscope study of the macrogametes of E. intestinalis (Coccidia). Tsitologiya 11 (6), 700–706 (1969).
Wohlfarth-Bottermann, K. E.: Kontrastierung tierischer Zellen und Gewebe im Rahmen ihrer elektronenmikroskopischen Untersuchung an ultradünnen Schnitten. Naturwissenschaften 44, 287–288 (1957).
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Supported in part by the Deutsche Forschungsgemeinschaft, by research grant GB-.8252 from the National Science Foundation and by a stipend to D. M. Hammond from the Heinrich Hertz-Stiftung.
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Scholtyseck, E., Hammond, D.M. Electron microscope studies of macrogametes and fertilization in Eimeria bovis . Z. F. Parasitenkunde 34, 310–318 (1970). https://doi.org/10.1007/BF00260299
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DOI: https://doi.org/10.1007/BF00260299