Summary
An inhibitory activity to (Na,K)ATPase was found in cell extracts of the brine shrimp, Artemia salina, irrespective of its developmental stages. Organic solvent extraction together with gas chromatographic analysis reveals that the inhibitory activity is due to long-chain, non-esterified fatty acids and their derivatives. Unsaturated fatty acids, especially with cis-configuration, are more effective in inhibition than saturated ones.
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Abbreviations
- ATPase:
-
adenosine triphosphatase
- EDTA:
-
ethylenediamine-tetraacetate
- TLC:
-
thin-layer chromatography
References
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Morohashi, M., Tsuchiya, K., Mita, T. et al. Identification of (Na,K)ATPase inhibitor in brine shrimp, Artemia salina, as long-chain fatty acids. J Comp Physiol B 161, 69–72 (1991). https://doi.org/10.1007/BF00258748
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DOI: https://doi.org/10.1007/BF00258748