Summary
A mathematical model simulating the behaviour or Streptomyces aureofaciens in batch culture under conditions when tetracyclines are synthesized in excessive amounts has been formulated. The response of the mathematical model to the experimental conditions applied corresponds with data obtained in the experiments. The mathematical model demonstrated that the level of tetracycline production is determined during the period of culture growth beginning with exhaustion of inorganic phosphate from the medium and ending with inhibition of the synthesis of enzymes caused by the synthesized tetracyclines. Further tetracycline synthesis is then proportional to the amount of enzymes synthesized in this interval.
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Abbreviations
- E :
-
Activity of ACT-oxygenase (10×nkat/g)
- P :
-
Product concentration (mg/l)
- k 1-k 6 :
-
Rate constants
- K S :
-
Saturation constant (g sugar/l)
- K I1 :
-
Inhibition constant (mg product/l)
- K I2 :
-
Inhibition constant (mM phosphate/l)
- K I3 :
-
Inhibition constant (mg product/l)
- S 1 :
-
Substrate sucrose (g sugar/l)
- S 2 :
-
Substrate concentration — phosphate (mM/l)
- r P :
-
Specific rate of product formation (mg product/g · h)
- r E :
-
Specific rate of enzyme synthesis (10×nkat/g2 · h), Expressed by activity units
- t :
-
Cultivation time (hour)
- X :
-
Biomass dry weight (g/l)
- Y S/X :
-
Yield coefficient
- μ:
-
Specific growth rate (h-1)
References
Běhal V (1980) Oligoketide-synthesizing enzymes. In: Krumphanzl V, Sikyta B, Vaněk Z (eds) Overproduction of microbial metabolites. Academic Press, London, pp 301–309
Běhal V, Vaněk Z (1970) Regulation of biosynthesis of secondary metabolites. XII. Acetyl CoA carboxylase in S. aureofaciens. Folia Microbiol 15:354–357
BěhalV, PodojilM, HošťálekZ, VaněkZ, LynenF (1974) Regulation of biosynthesis of secondary metabolites. XVI. Origin of terminal group of tetracyclines. Folia Microbiol 19:146–150
Běhal V, Jechová V, Vaněk Z, Hošťálek Z (1977) Alternate pathways of malonyl CoA formation in Streptomyces aureofaciens. Phytochemistry 16:347–350
Běhal V, Vaněk Z, Hošťálek Z, Ramadan A (1979a) Synthesis and degradation of proteins and DNA during growth of Streptomyces aureofaciens. Folia Microbiol 24:211–216
Běhal V, Hošťálek Z, Vaněk Z (1979b) Anhydrotetracycline oxygenase activity and biosynthesis of tetracyclines in Streptomyces aureofaciens. Biotechnol Lett 1:177–182
Běhal V, Grégrová-Prušáková J, Hošťálek Z (1982) Effect of phosphate and benzylthiocyanate on the activity of anhydrotetracycline oxygenase in Streptomyces aureofaciens. Folia Microbiol 27:102–106
Froyshof O (1977) The production of bacitracin synthetase by Bacillus licheniformis ATCC 10716. FEBS Lett 81:315–318
Hošťálek Z, Tintěrová M, Jechová V, Blumauerová M, Suchý J, Vaněk Z (1969) Regulation of biosynthesis of secondary metabolites. I. Biosynthesis of chlortetracycline and tricarboxylic acid cycle activity. Biotechnol Bioeng 9:539–548
Hošťálek Z, Běhal V, Novotná J, Erban V, Čurdová E, Jechová V (1982) Regulation of expression of chlortetracycline synthesis in Streptomyces aureofaciens. In: Krumphanzl V, Sikyta B, Vaněk Z (eds) Overproduction of microbial metabolites. Academic Press, London, pp 47–62
Lee SG, Lipmann F (1975) Tyrocidine synthetase system. Hash JH (ed) Methods in enzymology XLIII, Academic Press, New York San Franciso London, pp 585–602
Votruba J (1982) Practical aspects of the mathematical modeling of fermentation processes as a method of description, simulation, identification on optimization. Acta Biotechnol 2:119–126
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Votruba, J., Běhal, V. A mathematical model of kinetics of the biosynthesis of tetracyclines. Appl Microbiol Biotechnol 19, 153–156 (1984). https://doi.org/10.1007/BF00256446
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DOI: https://doi.org/10.1007/BF00256446