Abstract
Sedimentation equilibrium and low-angle laser-light scattering were used to determine the molar mass of the glycoprotein moieties in the complexes of sodium dodecyl sulphate with the human platelet membrane glycoproteins IIb (GPIIb), IIIa (GPIIIa), and the α (GPIIbα) and β (GPIIbβ) subunits of GPIIb. The values obtained by both procedures, except those for GPIIb, agree within experimental error with those calculated from their chemical composition: GPIIbα (114,000 g mol-1), GPIIbβ (22,200 g mol-1), and GPIIIa (91,500 g mol-1). The molar mass of GPIIb determined by light scattering (142,000 g mol-1) and sedimentation equilibrium at different solvent densities (134,000 g mol-1) also agree, within experimental error, with the values calculated either from its chemical composition (136,500 g mol-1) or from the sum of the molar masses of its subunits. However the molar mass determined by sedimentation equilibrium at constant solvent density, is consistently underestimated (116,000 g mol-1).
High-performance size-exclusion chromatography in sodium dodecyl sulphate solutions overestimates the molar mass of these glycoproteins and their Stokes radii, and therefore the maximal frictional ratios derived from them.
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Abbreviations
- GPIIb:
-
glycoprotein IIb
- GPIIIa:
-
glycoprotein IIIa
- GPIIbα and GPIIbβ:
-
α and β subunits of GPIIb, respectively
- CM-GPIIbα:
-
CM-GPIIbβ, and CM-GPIIIa, totally reduced and carboxymethylated forms of GPIIbα, GPIIbβ, and GPIIIa, respectively
- SDS:
-
sodium dodecyl sulphate
- eosin-ITC:
-
eosin-5-isothiocyanate
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Usobiaga, P., Calvete, J.J., Saíz, J.L. et al. Molecular characterization of human platelet glycoproteins IIIa and IIb and the subunits of the latter. Eur Biophys J 14, 211–218 (1987). https://doi.org/10.1007/BF00256354
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DOI: https://doi.org/10.1007/BF00256354