Summary
The phosphoenolpyruvate carboxylase gene (ppc) of Escherichia coli K-12 was cloned on the multi-copy plasmid pLG339. Plasmid pST101, which carried a 4.3-kb SalI fragment, was introduced into Serratia marcescens T-1165, which carried the seven regulatory mutations for three aspartokinases and two homoserine dehydrogenases. Strain T-1165[pST101] produced phosphoenolpyruvate carboxylase at a rate 26 times higher than the control strain T-1165[pLG339]. While T-1165[pST101] produced 63 mg/ml l-threonine in a medium containing sucrose and urea, whereas T-1165 only produced 52 mg/ml.
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Sugita, T., Komatsubara, S. Construction of a threonine-hyperproducing strain of Serratia marcescens by amplifying the phosphoenolpyruvate carboxylase gene. Appl Microbiol Biotechnol 30, 290–293 (1989). https://doi.org/10.1007/BF00256220
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DOI: https://doi.org/10.1007/BF00256220