Abstract
2D NMR spectroscopy and J coupling constant analysis are applied to resolve the structure of two photoproducts of thymidylyl-(3′→5′)-thymidine. These products are cyclobutane type thymine dimers possessing the cis-syn (the predominant one) and trans-syn geometry. The cis-syn is formed in an ANTI-ANTI conformation about the N-glycosyl linkages and resembles the normal base-stacked configuration. The glycosidic conformation in solution of the 5′ terminal fragment differs from the crystal in which the less common SYN conformation is observed. In this isomer only the sugar pucker of the 3′ terminal fragment is changed substantially with respect to the dinucleotide. The trans-syn isomer is formed in a SYN-ANTI glycosidic conformation. In this isomer the sugar puckers of both deoxyribose rings are affected and a preference for a pure 2′-endo conformation is observed.
Similar content being viewed by others
Abbreviations
- dTpdT:
-
2′-deoxythymidylyl-(3′→5′)-2′-deoxythymidine
- dTp[]dT:
-
cyclobutane type photodimers of dTpdT
- dTp- and dTp[]-:
-
their 5' terminal fragments (fragment A)
- -pdT and-[]pdT:
-
their 3′ terminal fragments (fragment B)
- RP-HPLC:
-
reversed-phase high-performance liquid chromatography
- COSY:
-
two-dimensional correlated spectroscopy
- 2D NOE:
-
two-dimensional nuclear Overhauser spectroscopy
References
Antona C (1982) Conformational analysis of nucleic acids. Determination of backbone geometry of single-helical RNA and DNA in aqueous solution. Recl Trav Chim Pays-Bas 101:413–433
Ben-Hur E, Ben-Ishai R (1968) Trans-syn thymine dimers in ultraviolet-irradiated denaturated-DNA: identification and photoreactivability. Biochim Biophys Acta 166:9–15
Beukers R, Ijlstra J, Berends W (1960) The effect of ultraviolet light on some components of the nucleic acids. Recl Trav Chim 79:101–104
Blackburn GM, Davis RJH (1967) Photochemistry of nucleic acids. III. The structure of DNA-derived thymine photodimer. J Am Chem Soc 89:5941–5945
Boelens R, Scheek RM, Dijkstra K, Kaptein R (1985) Sequential assignment of imino- and amino-protein resonances in 1H NMR spectra of oligonucleotides by two-dimensional NMR spectroscopy. Application to a lac operator fragment. J Mag Res 62:378–386
Cadet J, Voituriez L, Hruska FE, Grand A (1985) Crystal structure of the cis-syn photodimer of thymidylyl-(3′→5′)-thymidine cyanoethyl ester. Biopolymers 24:897–903
Camerman N, Camerman A (1970) Crystal and molecular structure of photodimer A of 1,3-dimethylthymine (the isomer in irradiated deoxyribonucleic acid). J Am Chem Soc 92:2523–2527
Demidov VV, Potaman VN (1984) High-performance liquid chromatography of nucleic acid components. I. Thymine dimers in short oligonucleotides. J Chromatogr 285:135–142
Feigon J, Wright JM, Leupin W, Denny WA, Kearns DR (1982) Use of two-dimensional NMR in the study of a double-stranded DNA decamer. J Am Chem Soc 104: 5540–5541
Haasnoot CAG, de Leeuw FAAM, de Leeuw HPM, Altona C (1980) The relationship between proton-proton NMR coupling constants and substituent electronegativities. I. An empirical generalization of the Karplus equation. Tetrahedron 36:2783–2792
Haasnoot CAG, de Leeuw FAAM, de Leeuw HPM, Altona C (1981) The relationship between proton-proton NMR coupling constants and substituent electronegativities. II. Conformational analysis of the sugar ring in nucleosides and nucleotides in solution using a generalized Karplus equation. Org Magn Reson 15:43–52
Hare DR, Wemmer DE, Chou S-H, Drobny G, Reid BR (1983) Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methods. J Mol Biol 171:319–336
Hollis DP, Wang SY (1967) Structure of homodimers of thymine and dimethylthymine. A nuclear magnetic resonance study. J Org Chem 32:1620–1622
Hruska FE, Wood DJ, Ogilvie KK, Charlton JL (1975) A proton magnetic resonance study of the ultraviolet photoproduct of d(TpT) in aqueous solution. Can J Chem 53:1193–1203
Johns HE, Pearson ML, LeBlanc JC, Helleiner CW (1964) The ultraviolet photochemistry of thymidylyl-(3′→5′)-thymidine. J Mol Biol 9:503–524
Lui F-T, Yang NC (1978) Photochemistry of cytosine derivatives. I. Photochemistry of thymidylyl-(3′→5′)-deoxycytidine. Biochemistry 17:4865–4876
Rance M, Sørensen OW, Leupin W, Kogler M. Wuthrich K, Ernst RR (1985) Uniform excitation of multiple-quantum coherence. Application to two-dimensional double-quantum spectroscopy. J Magn Res 61:67–80
Rao SN, Keepers JW, Kollman P (1984) The structure of d(CGCGAAT[]TCGCG)*#x002A;d(CGCGAATTCGCG): the incorporation of a thymine photodimer into a B-DNA helix. Nucleic Acids Res 12:4789–4809
Scheek RM, Russo N, Boelens R, Kaptein R (1983) Sequential resonance assignment in DNA 1H NMR spectra by two-dimensional NOE spectroscopy. J Am Chem Soc 105:2914–2916
Scheek RM, Boelens R, Russo N, van Boom JH, Kaptein R (1984) Sequential resonance assignment in 1H NMR spectra of oligonucleotides by two-dimensional NMR spectroscopy. Biochemistry 23:1371–1376
Wacker A (1961) Strahlenchemische Veränderungen von Pyrimidinen in Vivo und in Vitro. J Chim Phys 58:1041–1045
Wacker A, Dellweg H, Weinblum D (1960) Strahlenchemische Veränderung der Bakterien-Desoxyribonucleinsäure in vivo. Naturwissenschaften 20:477
Weinblum D (1967) Characterization of the properties of isomeric thymine dimers. Biochem Biophys Res Commun 27:384–390
Weinblum D, Johns HE (1966) Isolation and properties of isomeric thymine dimers. Biochim Biophys Acta 114: 450–459
Wood DJ, Hruska FE, Ogilvie KK (1974) Proton magnetic resonance studies of 2′-deoxythymidine, its 3′- and 5′-monophosphates and 2′-deoxythymidylyl-(3′←5′)-2′-deoxythymidine in aqueous solution. Can J Chem 52:3353–3366
Wulff DL (1963) The role of thymine dimer in the photoinactivation of the Bacteriophage T4 299–1. J Mol Biol 7:431–441
Wulff DL, Rupert CS (1962) Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from Baker's yeast. Biochem Biophys Res Commun 7:237–240
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kemmink, J., Boelens, R. & Kaptein, R. Two-dimensional 1H NMR study of two cyclobutane type photodimers of thymidylyl-(3′→5′)-thymidine. Eur Biophys J 14, 293–299 (1987). https://doi.org/10.1007/BF00254894
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00254894