Abstract
Possibilities for the use of fluorescence correlation spectroscopy in the nanosecond time range are demonstrated. The experiment is based on a cw argon ion laser, a microfluorimeter, two photon detectors, and a time-to-analog converting system. Experiments using solutions of rhodamine 6G and pyronine G in water at concentrations of about 20 molecules per sample volume are reported. The photon anticorrelation component decaying with a time constant close to the excited state lifetime was observed.
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Kask, P., Piksarv, P. & Mets, Ü. Fluorescence correlation spectroscopy in the nanosecond time range: Photon antibunching in dye fluorescence. Eur Biophys J 12, 163–166 (1985). https://doi.org/10.1007/BF00254074
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DOI: https://doi.org/10.1007/BF00254074