Summary
The gene for β-CGTase from an alkalophilic bacterium, Bacillus sp. #1011, was cloned in an Escherichia coli phage λ D69 and recloned in an E. coli plasmid pBR322 and a B. subtilis plasmid pUB110. An E. coli recombinant plasmid pTUE202 and a B. subtilis plasmid pTUB703 were selected from ten plasmids, because the transformants by each of the two plasmids produced the highest amount of extracellular β-CGTase in each strain. The plasmids were stably maintained and expressed in each bacterial strain. A common DNA region of approximately 2.5 kb was defined in the ten plasmids, and the enzymatic activity was lost when a part of the common region was deleted. The major product of hydrolysis from starch by the β-CGTases of E. coli [pTUB202] and B. subtilis [pTUB703] was β-CD as in the case of the enzyme of the parental Bacillus sp. #1011.
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Abbreviations
- β-CGTase:
-
β-cyclodextrin synthetase
- β-CD:
-
β-cyclodextrin
- α-CD:
-
α-cyclodextrin
- γ-CD:
-
γ-cyclodextrin
- []:
-
designates plasmid-carrier state
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Kimura, K., Takano, T. & Yamane, K. Molecular cloning of the β-cyclodextrin synthetase gene from an alkalophilic Bacillus and its expression in Escherichia coli and Bacillus subtilis . Appl Microbiol Biotechnol 26, 149–153 (1987). https://doi.org/10.1007/BF00253900
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DOI: https://doi.org/10.1007/BF00253900