Summary
The fermenter-growth conditions for a high yield of non-dissociated strain 19 Brucella abortus are described allowing a yield of close to 200×109 viable bacteria ml-1 of culture medium in less than 48 h. Details are also given of an improved growth-medium which may be sterilized by filtration or more advantageously by autoclaving in situ.
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References
Alton GG, Corner LA, Plackett P (1980) Vaccination of pregnant cows with low doses of Brucella abortus strain 19 vaccine. Aust Vet J 56:369–372
Angus RD (1984) An evaluation of the stability of Brucella abortus strain 19 reduced dosage lyophilized vaccines produced by different methods. Dev Biol Stand 56:659–678
Boyce KJ, Edgar AW (1966) Production of freeze-dried Brucella abortus strain 19 vaccine using cells produced by continuous culture. J Appl Bacteriol 29:401–408
Cameron J (1967) Brucella vaccines. Brit Pat 1 091 595
Davies G, Cocks E, Hebert N (1980) Brucella abortus (strain 19) vaccine: (a) determination of the minimum protective dose in cattle; (b) the effect of vaccinating calves previously inoculated with anti-Brucella abortus serum. J Biol Stand 8:165–175
Edwards DC (1960) The growth and toxin production of Corynebacterium diphtheriae in submerged culture. J General Microbiol 22:698–704
Fredette V, Vinet G (1952) Production en sac de cellophane de toxines tétaniques renfermant au moins 600 000 doses mortelles (cobaye) par millilitre. Can J Med Sci 30:155
Gerhardt P, Gee LL (1946) Brucella suis in aerated broth culture. I. Preliminary studies on growth assays, inoculum and growth characteristics in an improved medium. J Bacteriol 52:261–269
Hauschild AHW, Pivnick H (1961) Continuous culture of Brucella abortus S 19. Can J Microbiol 7:491–505
Henry BS (1933) Dissociation of the genus Brucella. J Infect Dis 52:374–402
Seligmann Jr EB, Farber JF (1976) Use of U.S. opacity standard. J Biol Stand 4:127–130
Sterne M (1958) The growth of Brucella abortus strain 19 in aerated dialysed media. J Gen Microbiol 18:747–750
van Drimmelen GC (1956) Strain 19 Brucella vaccine. I. Production of vaccine by the shake-flask technique. Onderstepoort J Vet Res 27:205–214
van Drimmelen GC (1958) Strain 19 Brucella vaccine. V. Mass production in the Brucella vortex aerated culture apparatus. Onderstepoort J Vet Res 27:539–547
Zavanella M, Nigrelli A, Ciuchini F, Sala V (1984) Production and control of freeze-dried Brucella abortus strain 19 vaccine by using cells produced by fermentation units. Appl Microbiol Biotechnol 19:229–231
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Rousseau, P., Ricard, C. & Cameron, J. Improvements in the fermenter growth of Brucella abortus strain 19. Appl Microbiol Biotechnol 26, 422–426 (1987). https://doi.org/10.1007/BF00253525
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DOI: https://doi.org/10.1007/BF00253525