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Cloning and expression in Escherichia coli of a Thermoanaerobacter cellulolyticus gene coding for heat-stable β-glucanase

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Summary

A 4.8 kb HindIII fragment of Thermoanaerobacter cellulolyticus DNA cloned in Escherichia coli was shown to direct the synthesis of β-glucanase. The enzyme produced by the transformant was extremely heat-stable and the optimum temperature for the enzyme reaction was 80°C. The cloned enzyme could hydrolyse carboxymethyl cellulose and lichenan, but could not digest laminarin, xylan and cellobiose. Although T. cellulolyticus secreted cellulase(s) into the medium, most of the cloned enzyme activity was detected only in cytoplasm in the recombinant clone.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan

    Hiroyuki Honda, Hiroshi Naito, Masahito Taya, Shinji Iijima & Takeshi Kobayashi

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  1. Hiroyuki Honda
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  2. Hiroshi Naito
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  3. Masahito Taya
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  4. Shinji Iijima
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  5. Takeshi Kobayashi
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Honda, H., Naito, H., Taya, M. et al. Cloning and expression in Escherichia coli of a Thermoanaerobacter cellulolyticus gene coding for heat-stable β-glucanase. Appl Microbiol Biotechnol 25, 480–483 (1987). https://doi.org/10.1007/BF00253323

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  • DOI: https://doi.org/10.1007/BF00253323

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