Skip to main content
SpringerLink
Log in

Measurement of the partial pressure of dissolved CO2 for estimating the concentration of Streptococcus thermophilus in coculture with Lactobacillus bulgaricus

  • Applied Microbiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

The use of mixed cultures in the food industry is hindered by the lack of rapid and specific measurement techniques. The coculture of Streptococcus thermophilus and Lactobacillus bulgaricus, used for producing starters for yoghurt production, is a simple model of a mixed culture.

After verifying that Streptococcus thermophilus specifically degraded urea, we attempted to correlate the cell concentration of the species first with urease activity and then with the rate of CO2 production from urea in the medium. The measurement was performed in real time with a specific electrode for dissolved CO2. The results obtained with the method have the same uncertainy as those obtained by cell counts. The estimation is valid between 107 and 109 cells/ml.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Accolas JP, Bloquel R, Didienne R, Régnier J (1977) Propriétés acidifiantes des bactéries lactiques thermophiles en relation avec la fabrication du yoghourt. Le Lait 57:1–23

    Article  CAS  Google Scholar 

  • Bautista ES, Dahiya RS, Speck ML (1966) Identification of compounds causing symbiotic growth of Streptococcus thermophilus and Lactobacillus bulgaricus in milk. J Dairy Res 33:299–307

    Article  CAS  Google Scholar 

  • Barrère C, Ducasse M, Vigne C, Vidal M, Tallec A (1976) Dosage du CO2 dans les vins. Vignes et vins 273:31–38

    Google Scholar 

  • Bracquart P, Lorient D (1977) Effet des acides aminés sur la croissance de Streptococcus thermophilus. Milchwissen-schaft 32:221–224

    CAS  Google Scholar 

  • Breed RS (1911) The determination of the number of bacteria in milk by direct microscopic examination. Zentralblatt für Bakt Part II 30:227

    Google Scholar 

  • Cantéri G (1984) Utilization of a specific probe for determination of carbon dioxide produced by lactic acid bacteria. Bio-Science 14:7–10

    Google Scholar 

  • Desmazeaud MJ, Hermier J (1972) Isolement et détermination de la composition qualitative des peptides issus de la caséine, stimulant la croissance de Streptococcus thermophilus. Eur J Biochem 28:190–198

    Article  CAS  Google Scholar 

  • Driessen FM, Kingma F, Stadhouders J (1982) Evidence that Lactobacillus bulgaricus in yogurt is stimulated by carbon dioxide produced by Streptococcus thermophilus. Neth Milk Dairy J 36:135–144

    Google Scholar 

  • Farrow JAE, Collins MD (1984) DNA base composition, DNA-DNA homology and long chain fatty acid studies on Streptococcus thermophilus and Streptococcus salivarius. J Gen Microbiol 130:357–362

    CAS  PubMed  Google Scholar 

  • Galesloot TE, Hassing F, Veringa HA (1968) Symbiosis in yoghurt (I) Stimulation of Lactobacillus bulgaricus by a factor produced by Streptococcus thermophilus. Neth Milk Dairy J 22:50–62

    CAS  Google Scholar 

  • Hugenholtz J, Velkamp H (1985) Competition between strains of Streptococcus cremoris. TEMS Microbiology Letters ecology 31:57–62

    Article  Google Scholar 

  • Lonvaud-Funel A, Matsumoto N (1979) Le coefficient de solubilité du gaz carbonique dans les vins. Vitis 18:137–147

    CAS  Google Scholar 

  • Lopez ME (1984) Selectivity of the potentiometric carbon dioxide gas-sensing electrode. Anal Chem 56:2360–2366

    Article  CAS  Google Scholar 

  • Miozzari GF, Niederberger P, Hütter R (1978) Permeabilisation of microorganisms by triton X-100. Analytical Biochem 90:220–233

    Article  CAS  Google Scholar 

  • Moon NJ, Reinbold GW (1976) Commensalism and competition in mixed cultures of Lactobacillus bulgaricus and Streptoccus thermophilus. J Milk Food Technol 39:337–341

    Article  Google Scholar 

  • Nelson GEM, Peterson RE, Cigler A (1973) Serine deshydratase from bacteria. J Appl Bacteriol 36:245–247

    Article  CAS  Google Scholar 

  • Pette JW, Lolkema H (1950) Yoghurt (I) Symbiosis and antisymbiosis in mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus. Neth Milk Dairy J 6:209–224

    Google Scholar 

  • Plaquet R, Leflon F (1975) Automatisation du, dosage de l'ammoniaque per la réaction de Nessler. Bulletin de la Societé de Pharmacie de Lille 1:45–51

    Google Scholar 

  • Shoda M, Ishikawa Y (1981) Carbon dioxide sensor for fermentation systems. Biotechnol Bioeng 23:461–466

    Article  CAS  Google Scholar 

  • Tayeb J, Bouillanne C, Desmazeaud MJ (1984) Computerized control of growth with temperature in a mixed culture of lactic acid bacteria. J Ferment Technol 62:461–470

    Google Scholar 

  • Tinson W, Broome MC, Hillier AJ, Jago GR (1982) Metabolism of Streptococcus thermophilus production of CO2 and NH3 from urea. Aust J Dairy Technol 37:14–16

    CAS  Google Scholar 

  • Veringa HA, Galesloot TE, Davelaar H (1968) Symbiosis in yoghurt (II) Isolation and identification of a growth factor for Lactobacillus bulgaricus produced by Streptococcus thermophilus. Neth Milk Dairy J 22:114–120

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Génie des Procédés Biotechnologiques alimentaires, I.N.R.A., Route de Thiverval, F-78 850, Grignon, France

    H. E. Spinnler & G. Corrieu

  2. Laboratoire de Microbiologie laitière, I.N.R.A., F-78 350, Jouy-en-Josas, France

    C. Bouillanne & M. J. Desmazeaud

Authors
  1. H. E. Spinnler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Bouillanne
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. J. Desmazeaud
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Corrieu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Spinnler, H.E., Bouillanne, C., Desmazeaud, M.J. et al. Measurement of the partial pressure of dissolved CO2 for estimating the concentration of Streptococcus thermophilus in coculture with Lactobacillus bulgaricus . Appl Microbiol Biotechnol 25, 464–470 (1987). https://doi.org/10.1007/BF00253320

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00253320

Keywords

Search

Navigation