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A cellular analysis of long-term haematopoietic damage in mice after repeated treatment with cyclophosphamide

  • Original Articles
  • Cyclophosphamide, Haematopoiesis
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Summary

Following repeated treatment of mice with cyclophosphamide (5×200 mg/kg) it was found that slight, but significant, residual marrow damage persisted for at least half the lifespan of the animals. This long-term damage occurred despite preferential sparing of those multipotential haematopoietic cells (CFU-S) that had a high self-renewal capacity after each step of the multistep regimen and despite a smaller CFU-S kill after each successive dose. The damage was characterized by low mean numbers of CFU-S and stromal colony-forming cells (CFU-F) which were around 70% of control values.

Examination of individual animals revealed that the majority had slightly subnormal numbers of CFU-S and CFU-F, with only a few suffering a more severe injury, including 8% of mice with clinical hypoplasia or myelodysplasia.

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Authors and Affiliations

  1. Paterson Laboratories, Christie Hospital and Holt Radium Institute, M20 9BX, Withington, Manchester, England

    Graham Molineux, Chengxiong Xu, Jolyon Hendry & Nydia Garcia Testa

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  1. Graham Molineux
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  2. Chengxiong Xu
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  3. Jolyon Hendry
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  4. Nydia Garcia Testa
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Molineux, G., Xu, C., Hendry, J. et al. A cellular analysis of long-term haematopoietic damage in mice after repeated treatment with cyclophosphamide. Cancer Chemother. Pharmacol. 18, 11–16 (1986). https://doi.org/10.1007/BF00253055

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  • DOI: https://doi.org/10.1007/BF00253055

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