Summary
Renal function was examined with micropuncture methods in the insulin-treated streptozotocin-diabetic rat. Kidney glomerular filtration rate was significantly higher in the diabetic rats (1.21 ml/min) than in the control group (0.84 ml/min) Nephron glomerular filtration rate increased in proportion to the rise in kidney glomerular filtration rate (diabetic rats: 37.0 nl/min; control rats: 27.9 nl/min). Likewise renal plasma flow was significantly higher in the diabetic rats (4.1 ml/min) than in the control group (3.0 ml/min). Glomerular capillary pressure was identical in both groups (56.0 and 56.0 mmHg, respectively). The proximal intratubular pressure was significantly reduced in the diabetic rats (10.4 mmHg; control value: 12.5 mmHg). The effective glomerular ultrafiltration coefficient was slightly but not significantly higher in the diabetic rats (0.027 nl s-1mmHg-1) than in the control group (0.023 nl s-1mmHg-1). Kidney weight was significantly higher in the diabetic rats (1.15 g; control rats: 0.96 g) while body weight was similar in both groups (diabetic rats: 232 g; control rats: 238 g). Calculations indicate that the increases in transglomerular hydraulic pressure, renal plasma flow and ultrafiltration co-efficient of the glomerular membrane contribute about equally to the rise in glomerular filtration rate. The increases in the values of the determinants of glomerular filtration rate may be the result of renal hypertrophy. These studies suggest that this model provides a useful method for investigating kidney function in diabetes, which may have relevance for our understanding of the kidney abnormalities in human diabetes.
Article PDF
Similar content being viewed by others
References
Andreucci VE (1978) Manual of renal micropuncture. Idelson, Naples
Arendhorst WJ, Gottschalk CW (1980) Glomerular ultrafiltration dynamics: euvolemic and plasma volume-expanded rats. Am J Physiol 239:F171-F186
Azar S, Johnson MA, Hertel B, Tobian L (1977) Single-nephron pressures, flows, and resistances in hypertensive kidneys with nephrosclerosis. Kidney Int 12:28–40
Barratt LJ, Wallin JD, Rector FC, Seldin DW (1973) Influence of volume expansion on single-nephron filtration rate and plasma flow in the rat. Am J Physiol 224:643–650
Blantz RC, Israelit AH, Rector FC, Seldin DW (1972) Relation of distal tubular NaCl delivery and glomerular hydrostatic pressure. Kidney Int 2:22–32
Brenner BM, Humes HD (1977) Mechanisms of glomerular ultrafiltration. N Engl J Med 297:148–154
Christiansen JS, Gammelgaard J, Frandsen M, Parving H -H. Increased kidney size, glomerular filtration rate and renal plasma flow in short-term insulin-dependent diabetics. Diabetologia 20:451–456
Daugharty TM, Ueki IF, Nicholas DP, Brenner BM (1972) Comparative renal effects of isoncotic and colloid-free volume expansion in the rat. Am J Physiol 222:225–235
Deen WM, Maddox DA, Robertson CR, Brenner BM (1974) Dynamics of glomerular ultrafiltration in the rat. VII. Response to reduced renal mass. Am J Physiol 227:556–562
Deen WM, Robertson CR, Brenner BM (1972) A model of glomerular ultrafiltration in the rat. Am J Physiol 223: 1178–1183
Dev B, Drescher C, Schnermann J (1974) Resetting of tubuloglomerular feedback sensitivity by dietary salt intake. Pfluegers Arch 346:263–277
Ditzel J, Junker K (1972) Abnormal glomerular filtration rate, renal plasma flow and renal protein excretion in recent and short-term diabetics. Br Med J 2:13–19
Gertz KH, Mangos JA, Braun G, Pagel HD (1966) Pressure in the glomerular capillaries of the rat kidney and its relation to arterial blood pressure. Pfluegers Arch 288:369–374
Hansen AP (1972) Serum growth hormone patterns in juvenile diabetes. Dan Med Bull 19:3–32
Hayslett JP, Kashgarian M, Epstein FH (1968) Functional correlates of compensatory renal hypertrophy. J Clin Invest 47:774–782
Hostetter TH, Troy JL, Brenner BM (1981) Glomerular hemodynamics in experimental diabetes mellitus. Kidney Int 19: 410–415
Jensen PK, Steven K (1979) Influence of intratubular pressure on proximal tubular compliance and capillary diameter in the rat kidney. Pfluegers Arch 382:179–187
Kroustrup JP, Gundersen HJG, Osterby R (1977) Glomerular size and structure in diabetes mellitus. Diabetologia 13: 207–210
Michels L, Keane WF, Davidman M (1979) Glomerular permselectivity in alloxan diabetes (Abstract). Diabetes 28:388
Mogensen CE (1971) Glomerular filtration rate and renal plasma flow in short-term and long-term juvenile diabetes mellitus. Scand J Clin Lab Invest 28:91–100
Mogensen CE, Andersen MJF (1973) Increased kidney size and glomerular filtration rate in early juvenile diabetes. Diabetes 22:706–712
Mogensen CE, Andersen MJF (1975) Increased kidney size and glomerular filtration rate in untreated juvenile diabetics: Normalization by insulin treatment. Diabetologia 11:221–224
Olivetti G, Anversa P, Melissari M, Loud AV (1980) Morphometry of the renal corpuscle during postnatal growth and compensatory hypertrophy. Kidney Int 17:438–454
Ott CE, Marchand GR, Diaz-Buxo JA, Knox FG (1976) Determinants of glomerular filtration rate in the dog. Am J Physiol 231:235–239
Rasch R (1979) Control of blood glucose levels in streptozotocin diabetic rat using a long acting heat treated insulin. Diabetologia 16:185–190
Reubi FC (1953) Glomerular filtration rate, renal blood flow and blood viscosity during and after diabetic coma. Circ Res 1:410–416
Schor N, Ichikawa I, Brenner BM (1980) Glomerular adaptations to chronic dietary salt restrictions or excess. Am J Physiol 238:F428-F436
Seyer-Hansen K, Hansen J, Gundersen HJG (1980) Renal hypertrophy in experimental diabetes: A morphometric study. Diabetologia 18:501–506
Solomon S (1977) Developmental changes in nephron number, proximal tubular length and superficial nephron glomerular filtration rate of rats. J Physiol 272:573–589
Steven K (1974) Effect of peritubular infusion of angiotensin II on rat proximal nephron function. Kidney Int 6:73–80
Tucker BJ, Blantz RC (1977) Factors determining superficial nephron filtration in the mature, growing rat. Am J Physiol 232: 97–104
Tonder KH, Aukland K (1979) Glomerular capillary pressure in the rat. Acta Physiol Scand 109:93–95
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jensen, P.K., Christiansen, J.S., Steven, K. et al. Renal function in streptozotocin-diabetic rats. Diabetologia 21, 409–414 (1981). https://doi.org/10.1007/BF00252690
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00252690