Summary
The growth behaviour of Memnoniella echinata and Fusarium roseum was examined in slurry fermentation systems using untreated orange peel as substrate. A composite experiment was then designed to study the effect of orange peel initial concentration and the effect of the nitrogen: peel ratio on crude protein yidld (Y p ) and protein enrichment (Z p ) of the final biomass.
The more concentrated the peel slurry, the greater the substrate inhibitory effect on microbial growth becomes.
Finally, multiple regression technique allowed both the experimental values of Y p and Z p to be reconstructed with mean percentage errors smaller than 4% and 8%, respectively, and the optimal operating strategy for such SCP production process to be determined.
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References
AOAC (1975) Official methods of analysis, 12th edn. Horwitz W (ed), Washington DC
Braddock RJ (1980) Quality of citrus specialty products. In: Nagy S, Attaway JA (eds) Citrus nutrition and quality. ACS Symp. Series 143, American Chemical Society, Washington DC, pp 273–288
Davies OL (1956) The design and analysis of industrial experiments. Imperial Chemical Industries Ltd, London
Himmelblau DM (1970) Process analysis by statistical methods. J. Wiley, New York
Humphrey AE, Moreira A, Armiger W, Zabriskie D (1977) Production of single-cel protein from cellulose wastes. Biotechnol Bioeng Symp 7:45–64
Karapinar M, Okuyan M (1982) The utilisation of citrus waste as substrate for microbial protein production by the fungus Sporotrichum pulverulentum. J Chem Tech Biotechnol 32: 1055–1058
Kertesz ZI (1951) The pectic substance. Interscience Publishers Inc., New York
Knapp JS, Howell JA (1980) Solid-state fermentation. In: Wiseman A (ed) Topics in enzyme and fermentation biotechnology. Vol. 4. Ellis-Horwood Ltd, Chichester, pp 85–13
Labaneiah MEO, Abou-Donia SA, Mohamed MS, ElZalaki EM (1979) Utilization of citrus wastes for the production of fungal protein. J Food Technol 14: 95–100
Lane AG (1980) Production of aromatic acids during anaerobic digestion of citrus peel. J Chem Tech Biotechnol 30: 345–350
Lequerica JL, Lafuente B (1977) Aprovechaniento de subproductos citricos. II) Fermentacion en medio semisolido de corteza de naranja por Candida utilis. Rev Agroqim Technol Aliment 17: 71–78
Moo-Young M, Chahal DS, Vlach D (1978) Single cell protein from various chemically pretreated wood substrates using Chaetomium cellulolyticum. Biotechnol Bioeng 20: 107–118
Moo-Young M, Daugulis AJ, Chahal DS, MacDonald DG (1980) The Waterloo SCP process for feed/food production from biomass. In: Linko P, Mälkki Y, Olkku J, Larinkari J (eds) Food Process Engineering. Food processing systems. Vol. 1. Applied Science Publishers Ltd, London, pp 896–900
Moresi M (1984a) SCP production from acid hydrolyzate of orange peel. In: Alessi P, Kikic I (eds) Proceedings of the 4th Italian-Yugoslavian-Austrian Chemical Engineering Conference. Vol. 2, Grado (Italy) 24–26 September, pp 767–774
Moresi M (1984b) Economic study of concentrated citrus juice production. In: McKenna BM (ed) Engineering and Food. Vol. 2 — Processing applications. Chp. 41. Elsevier Applied Science Publishers, London, pp 975–991
Rossi J, Clementi F, Ribaldi M (1983) Fungal biomass from cellulosic materials: microbial screening and cultivation conditions. In: Proceedings of the 20th Nat. Congr. of the Italian Microbiology Society, Gardone Riviera (Italy), 12–15 May, in press
Rossi J, Clementi F (1984) Fungal biomass from cellulosic materials. II) Microbial selection and cultivation conditions for growth on citrus peel. In: Proceedings of the Workshop on “Prospects in the production of microbial biomass from agro-industrial waste and surplus” National Research Council of Italy. Special Grant IPRA, Manfredonia (Italy) 25–26 May, in press
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Clementi, F., Moresi, M. & Rossi, J. Effect of medium composition on microbial utilisation of citrus waste by mixed fungal culture. Appl Microbiol Biotechnol 22, 26–31 (1985). https://doi.org/10.1007/BF00252152
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DOI: https://doi.org/10.1007/BF00252152