Summary
Mutant strains of the N2-fixing cyanobacterium bacterium Anabaena variabilis resistant to 6-fluorotryptophan or to ethionine were isolated. Many of these strains liberated amino acids into their media in the absence of 6-fluorotryptophan and ethionine. Nitrogenase activity was higher in mutant strains than in the parent strain. Mutant strains were immobilised in calcium alginate and sustained photoproduction of amino acids has been demonstrated.
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Abbreviations
- ETH:
-
ethionine
- FT:
-
6-fluorotryptophan
- Hepes:
-
4-(2-hydroxyethyl)-1, piperazine ethanesulphonic acid
- PEP:
-
phosphoenolpyruvate
- DAHP:
-
3-deoxy-d-arabinoheptulosonate 7-phosphate
- chl a:
-
chlorophyll a
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Kerby, N.W., Niven, G.W., Rowell, P. et al. Photoproduction of amino acids by mutant strains of N2-fixing cyanobacteria. Appl Microbiol Biotechnol 25, 547–552 (1987). https://doi.org/10.1007/BF00252015
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DOI: https://doi.org/10.1007/BF00252015