Summary
3-Indoleacetic acid (IAA) and 3-indolelactic acid (ILA) have been identified as tryptophan (Trp) catabolites in Azotobacter vinelandii cultures. IAA production depends linearly on initial Trp concentration within the range 0–0.05% Trp, decreases in the presence of 10 mM 2-oxoglutarate and is always smaller than ILA production. Tryptophan aminotransferase activity, found in the cultures, could explain the first step of Trp transformation into IAA. In order to rationalize the formation of ILA, the presence of the enzyme indolelactate dehydrogenase in A. vinelandii is suggested.
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García-Tabares, F., Herraiz-Tomico, T., Amat-Guerri, F. et al. Production of 3-indoleacetic acid and 3-indolelactic acid in Azotobacter vinelandii cultures supplemented with tryptophan. Appl Microbiol Biotechnol 25, 502–506 (1987). https://doi.org/10.1007/BF00252007
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DOI: https://doi.org/10.1007/BF00252007