Summary
The effect of insulin in vitro on the fluidity of the human erythrocyte membrane in Type I (insulin-dependent) diabetic patients and healthy control subjects was investigated using a fluorescence technique. It was found that the addition of 10-9 mol/l porcine insulin significantly increased fluorescent probe lateral mobility in the membrane lipid layer but did not appear to produce any conformational changes of membrane proteins.
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Sauerheber RD, Lewis UJ, Esgate JA, Gordon LM (1980) Effect of calcium, insulin and growth hormone on membrane fluidity. Biochim Biophys Acta 597: 292–304
Gambhir KK, Archer JA, Carter L (1977) Insulin radioreceptor assay for human erythrocytes. Clin Chem 23: 1590–1595
Gambhir KK, Archer JA, Bradley CJ (1978) Characteristics of human erythrocyte insulin receptors. Diabetes 27: 701–708
Robinson TJ, Archer JA, Gambhir KK, Hollis VW Jr., Carter L, Bradley CJ (1979) Erythrocytes: a new cell type for the evaluation of insulin receptor defects in diabetic humans. Science 205: 200–202
Schmid-Schönbein M, Volger E (1976) Red-cell aggregation and red cell deformability in diabetes. Diabetes 25 (Suppl 2): 897–902
McMillan DE (1976) Plasma protein changes, blood viscosity and diabetic microangiopathy. Diabetes 25 (Suppl 2): 858–864
Barnes AJ, Locke P, Scudder PR, Dormandy TL, Dormandy JA, Slack J (1977) Is hyperviscosity a treatable component of diabetic microcirculatory disease? Lancet 2: 789–791
McMillan DE, Utterback NG, Puma JL (1978) Reduced erythrocyte deformability in diabetes. Diabetes 27: 895–901
Bureau M, Healy JC, Bourgoin D, Joly M (1980) Rheological hysteresis of blood at low shear rates. Biorheology 17: 191–203
Baba Y, Kai M, Kamada T, Setoyama S, Otsuji S (1979) Higher levels of erythrocyte membrane microviscosity in diabetes. Diabetes 28: 1138–1140
Galla H-J, Sackmann E (1974) Lateral diffusion in the hydrophobic region of membranes: use of pyrene excimers as optical probes. Biochim Biophys Acta 339: 103–115
Galla H-J, Luisetti J (1980) Lateral and transversal diffusion and phase transitions in erythrocyte membranes. An excimer fluorescence study. Biochim Biophys Acta 596: 108–117
Dodge JT, Mitchell C, Hanahan DJ (1963) The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys 100: 119–130
Eftink MR, Ghiron CA (1976) Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies. Biochemistry 15: 672–680
Luly P, Baldini P, Incerpi S, Tria E (1981) Insulin effect in vitro on human erythrocyte plasma membrane. Experientia 37: 431–433
Bailey IA, Garrat CJ, Wallace SM (1978) An effect of fluorescent probes and of insulin on the structure of adipocyte membranes. Biochem Soc Trans 6: 302–304
Luly P, Shinitzky M (1979) Gross structural changes in isolated liver cell plasma membranes upon binding of insulin. Biochemistry 18: 445–450
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Bryszewska, M., Leyko, W. Effect of insulin on human erythrocyte membrane fluidity in diabetes mellitus. Diabetologia 24, 311–313 (1983). https://doi.org/10.1007/BF00251814
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DOI: https://doi.org/10.1007/BF00251814