Abstract
The anti-human tumour monoclonal antibody 791T/36 was conjugated to the cyclic dianhydride of DTPA and radio-labelled with 111In. The labelling method proved to be both simple and reliable and would be suitable for routine clinical use. Subsequent characterisation of this radio-pharmaceutical in vitro and in tumour-bearing hosts gave a strong indication as to its suitability for clinical tumour localisation studies.
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Armitage NC, Perkins AC, Pimm MV, Farrands PA, Baldwin RW, Hardcastle JD (1984) The localisation of an anti-tumour monoclonal antibody (791T/36) in gastrointestinal tumours. Br J Surg 71:407–412
Berche C, Mach J-P, Lumbroso JD, Langlais C, Aubry F, Buchegger F, Carrel S, Rougier P, Parmentier C, Tubiana M (1982) Tomoscintigraphy for detecting gastrointestinal and medullary thyroid cancers: first clinical results using radiolabelled monoclonal antibodies against carcinoembryonic antigen. Br Med J 285:1447–1451
Eckelman WC, Karesh SH, Reba RC (1975) New compounds: fatty acid and long chain hydrocarbon derivatives containing a strong chelating agent. J Pharm Sci 64:704–706
Epenetos AA, Britton KE, Mather S, Shepherd J, Granowska M, Taylor-Papadimitriou J, Nimmon CC, Durbin H, Hawkins LE, Malpas JS, Bodmer WF (1982) Targeting of iodine-123-labelled tumour-associated monoclonal antibodies to ovarian, breast and gastrointestinal tumours. Lancet ii:999–1005
Fairweather DS, Bradwell AR, Dykes PW, Vaughan AT, Watson-James SF, Chandler S (1983) Improved tumour localisation using indium-111 labelled antibodies. Br Med J 287:167–170
Farrands PA, Perkins AC, Pimm MV, Hardy JG, Embleton MJ, Baldwin RW, Hardcastle JD (1982) Radioimmunodetection of human colorectal cancers by an anti-tumour monoclonal antibody. Lancet ii:397–400
Hnatowich DJ, Layne WW, Childs RL (1982) The preparation and labelling of DTPA coupled albumin. Int J Appl Radiat Isot 33:327–332
Krejcarek GE, Tucker KL (1977) Covalent attachment of chelating groups to macromolecules. Biochem Biophys Res Commun 77:581–585
Larson SM, Brown JP, Wright PW, Carrasquillo JA, Hellstrom I, Hellstrom KE (1983) Imaging of melanoma with I-131-labelled monoclonal antibodies. J Nucl Med 24:123–129
Mach J-P, Buchegger F, Forni M, Ritschard J, Berche C, Lumbroso J-D, Mchrege M, Giradet C, Accolla RS, Carrel S (1981) Use of radiolabelled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and tomoscintigraphy. Immunology Today 2:239–249
Moshakis V, McIlhinney RAJ, Raghaven D, Neville AM (1981) Localization of human tumour xenografts after IV administration of radiolabelled monoclonal antibodies. Br J Cancer 44:91–99
Perkins AC, Armitage NC, Hardy JG, Wastie ML, Pimm MV, Hardcastle JD (1984) Imaging bone diseases using a radiolabelled anti-osteosarcoma monoclonal antibody. Nucl Med Commun 5:234–235
Perkins AC, Whalley DR, Hardy JG (1984) Physical approach for the reduction of dual radionuclide image subtraction artefacts in immunoscintigraphy. Nucl Med Commun 5:501–512
Pimm MV, Baldwin RW (1984) Quantitative evaluation of the localization of a monoclonal antibody (791T/36) in human osteogenic sarcoma xenografts. Eur J Cancer Clin Oncol 20:315–324
Pimm MV, Embleton MJ, Perkins AC, Price MR, Robins RA, Robinson GR, Baldwin RW (1982) In vivo localisation of anti-osteogenic sarcoma 791T monoclonal antibody in osteogenic sarcoma xenografts. Int J Cancer 30:75–85
Pimm MV, Perkins AC, Baldwin RW (1984) Immunoscintigraphy of tumours: possibilities and limitations. behring Inst Mitt 74:61–71
Rainsbury RM, Ott RJ, Westwood JH, Kalirai TS, Coombes RC, McCrady VR, Neville AM, Gazel JC (1983) Localisation of metastatic breast carcinoma by a monoclonal antibody chelate labelled with indium-111. Lancet ii:934–938
Symonds EM, Perkins AC, Pimm MV, Baldwin RW, Hardy JG, Williams DA (in press) Clinical implications for immunoscintigraphy in patients with ovarian malignancy: a preliminary study using monoclonal antibody 791T/36. Br J Obstet Gynaecol
Williams MR, Perkins AC, Campbell FC, Pimm MV, Hardy JG, Wastie ML, Blamey RW, Bladwin RW (1984) The use of monoclonal antibody 791T/36 in the immunoscintigraphy of primary and metastatic carcinoma of the breast. Clin Oncol 10:375–381
Yeh SM, Sherman DG, Meares CF (1979) A new route to ‘bifunctional’ chelating agents: conversion of amino acids to analogs of ethylenedinitrilotetraacetic acid. Anal Biochem 100:152–159
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Perkins, A.C., Pimm, M.V. & Birch, M.K. The preparation and characterisation of 111In-labelled 791T/36 monoclonal antibody for tumour immunoscintigraphy. Eur J Nucl Med 10, 296–301 (1985). https://doi.org/10.1007/BF00251299
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DOI: https://doi.org/10.1007/BF00251299