Summary
Somatic hybrid plants, produced between Nicotiana rustica and N. tabacum by heterokaryon isolation and culture and also by mutant complementation, were examined regarding their ability to set seed. From a total of seventeen independent somatic hybrids, three were found to be partially self-fertile while the others did not set seed. Differences regarding the methods of hybrid selection, parental varieties and chloroplast composition of hybrids did not appear to be significant regarding the ability of plants to set seed. Much variation in fertility was observed in subsequent generations and by recurrent selection of the most fertile, over two generations, it was possible to increase the level of self-fertility in some of the progeny. One R2 derivative possessed approximately a tenfold higher level of self-fertility than it's somatic hybrid parent. The presence of genetic markers from both parents were observed in all progeny indicating their hybrid nature.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brink RA, Cooper DC (1941) Incomplete seed failure as a result of somatoplastic sterility. Genetics 26:487–505
Chaleff RS (1981) Genetics of higher plants: applications of cell culture. Cambridge University Press, Cambridge
Chupeau Y, Missonier C, Hommel MC, Goujaud J (1978) Somatic hybrid plants by fusion of protoplasts. Observations on the model system “Nicotiana glauca — Nicotiana langsdorffi”. Mol Gen Genet 165:239–245
Collins GB (1979) Cytogenetic techniques. In: Durbin RD (ed) Nicotiana: procedures for experimental use. US Dept Agric Tech Bull 1586, pp 17–27
Douglas GC, Wetter LR, Nakamura C, Ketter WA, Setterfield G (1981) Somatic hybridization between Nicotiana rustica and N. tabacum. 3. Biochemical, morphological and cytological analysis of somatic hybrids. Can J Bot 59:228–237
Douglas GC, Wetter LR, Keller WA, Setterfield G (1983) Production of sexual hybrids of Nicotiana rustica x N. tabacum and N. rustica x N. glutinosa via in vitro culture of fertilized ovules. Z Pflanzenzücht 90:116–129
Evans DA (1983) Protoplast fusion. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol I. Macmillan, London New York, pp 291–321
Gleba YY, Evans DA (1983) Genetic analysis of somatic hybrid plants. In: Evans DA, Sharp WR, Ammirato PV Yamada Y (eds) Handbook of plant cell culture, vol I. Macmillan, London New York, pp 322–357
Hamill JD, Patnaik G, Pental D, Cocking EC (1984) The culture of manually isolated heterokaryons of Nicotiana tabacum and Nicotiana rustica. Proc Indian Acad Sci (Plant Sci) 93:317–328
Iwai S, Nago T, Nakata K, Kawashima N, Matsyama S (1980) Expression of nuclear and chloroplastic genes coding for Fraction 1 protein in somatic hybrids of Nicotiana tabacum + rustica. Planta 147:414–417
Jinks JL, Caligari PDS, Ingram NR (1981) Gene transfer in Nicotiana rustica using irradiated pollen. Nature 291:586–588
Legg PD, Mann TJ (1961) A study of introgression of N. rustica germplasm into flue cured varieties of N. tabacum. Tob Sci 5:136–139
Murata M, Orton TJ (1983) Chromosome structural changes in cultured celery cells. In vitro 19:83–89
Murata M, Orton TJ (1984) Chromosome fusions in cultured cells of celery. Can J Genet Cytol 26:395–400
Pental D, Cocking EC (1985) Some theoretical and practical possibilities of plant genetic manipulation using protoplasts. Hereditas (Suppl) 3:83–93
Pental D, Hamill JD, Cocking EC (1984) Somatic hybridisation using a double mutant of Nicotiana tabacum. Heredity 53:79–83
Power JB, Berry SF, Chapman JV, Cocking EC (1980) Somatic hybridization of sexually incompatible Petunias: Petunia parodii, Petunia parviflora. Theor Appl Genet 57:1–4
Radhakrishna Murty B, Swaminathan MS (1957) Cytogenetic studies in derivatives of Nicotiana rustica x N. tabacum. Euphytica 6:227–236
Smith HH, Kao KN, Combatti NC (1976) Interspecific hybridization by protoplast fusion in Nicotiana. J Hered 67:123–128
Stavely JR (1979) Disease resistance. In: Durbin RD (ed) Nicotiana: procedures for experimental use. US Dept Agric Tech Bull 1586, pp 87–110
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Hamill, J.D., Pental, D. & Cocking, E.C. Analysis of fertility in somatic hybrids of Nicotiana rustica and N. tabacum and progeny over two sexual generations. Theoret. Appl. Genetics 71, 486–490 (1985). https://doi.org/10.1007/BF00251193
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00251193