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Heteroxanthinedemethylase, a new enzyme in the degradation of caffeine by Pseudomonas putida

  • Applied Microbiology
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Summary

In Pseudomonas putida a new enzyme has been detected, which converts heteroxanthine into xanthine by oxidative demethylation. Evidence is presented that this heteroxanthinedemethylase is not responsible for the demethylation of caffeine, supporting the hypothesis that specific enzymes catalyze caffeine demethylation in Pseudomonas putida.

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References

  • Birkett DJ, Grygiel JJ, Mines JO (1981) Metabolic disposition of methylxanthines in man. In: Theophylline and other Methylxanthines, N. Rietenbrock, Woodcock BG, Staib AH (eds), Vieweg Verlag, The Proceedings of an International Symposium, Frankfurt: 29th april and 3rd may 1981, pp 149–158

  • Blecher R, Lingens F (1977) The metabolism of caffeine by a Pseudomonas putida strain. Hoppe-Seyler's Z Physiol Chem 358:807–817

    Google Scholar 

  • Estabrook RW, Werringloer J (1972) The measurement of difference spectra: application to the cytochromes of microsomes. Methods in Enzymology LII, pp 212–228

    Google Scholar 

  • Farr DR (1982) Decaffeination method. Patentschrift (Switzerland) CH 626,781 Appl 78/1,205,03

  • Glück M, Lingens F (1987) Studies on the microbiological production of theobromine and heteroxanthine from caffeine. Appl Microbiol Biotechnol 25:334–340

    Google Scholar 

  • Hohnloser W, Osswald B, Lingens F (1980) Enzymological aspects of caffeine demethylation and formaldehyde oxidation in Pseudomonas putida C1. Hoppe-Seyler's Z Physiol Chem 361:1763–1766

    Google Scholar 

  • Kurtzmann RH, Schwimmer S, Heftmann E (1971) Caffeine metabolism by Penicillium roqueforti. Arch Biochem Biophys 147:109–113

    Google Scholar 

  • Kurtzmann RH, Schwimmer S (1972) Decaffeination of beverages. Patentschrift Appl 238,923,28 U.S. 3,749,584

  • Lismanis A, Alaine J, Vitols M (1973) Metabolism of caffeine in microorganisms. Uch Zap Latv Gos Univ 191:71–76

    Google Scholar 

  • Sauer M, Käppeli O, Fiechter A (1982) Comparison of the cytochrome P-450 containing monooxygenases originating from two different yeasts. Developments in Biochem 23:452–457

    Google Scholar 

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Hohenheim, Garbenstrasse 30, D-7000, Stuttgart 70, Federal Republic of Germany

    Michael Glück & Franz Lingens

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  1. Michael Glück
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  2. Franz Lingens
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Glück, M., Lingens, F. Heteroxanthinedemethylase, a new enzyme in the degradation of caffeine by Pseudomonas putida . Appl Microbiol Biotechnol 28, 59–62 (1988). https://doi.org/10.1007/BF00250499

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  • DOI: https://doi.org/10.1007/BF00250499

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