Summary
The uptake of d-{1-13C} xylose, the accumulation of intermediates and the distribution of the label in ethanol in Pichia stipitis under aerobic and anaerobic conditions were investigated by nuclear magnetic resonance spectroscopy. The rate-limiting step of d-xylose metabolism under aerobic conditions appeared to be uptake, whereas under anaerobic conditions it was the conversion of xylitol to xylulose. The yeast showed no preference to either the alpha-or beta-forms of d-xylose. Under anaerobic conditions only {2-13C{ ethanol was detected and this suggests that NADH but not NADPH was used as cofactor in the conversion of xylose to xylitol. d-Xylose is most likely metabolised by the pentose phosphate pathway in this yeast.
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Ligthelm, M.E., Prior, B.A., du Preez, J.C. et al. An investigation of d-{1-13C} xylose metabolism in Pichia stipitis under aerobic and anaerobic conditions. Appl Microbiol Biotechnol 28, 293–296 (1988). https://doi.org/10.1007/BF00250458
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DOI: https://doi.org/10.1007/BF00250458