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Histopathology and cell culture characteristics of liver cells from grc − and grc + rats given diethylnitrosamine

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Abstract

The histopathological response and cell culture characteristics of liver cells from the R16 (grc −) strain of rats, which carries an MHC-linked deletion, were examined one week after a single intraperitoneal injection of 200 mg/ kg body weight diethylnitrosamine (DEN) and were compared with the response of liver cells from wild type (grc+) rats. The DEN exposure induced hydropicl vacuolar changes in the parenchymal cells and a limited proliferation of oval cells in the periportal areas of the livers of both grc+ and grc rats. Primary culture of collagenase-digested livers consisted of parenchymal, bile ductular and oval-related cells as determined by cell-specific immunohistochemistry. Subpassaged cells from grc+ rats exhibited oval cell ultrastructural morphology, inducible histochemical staining for gammaglutamyl transpeptidase (GGT), and DEN-associated onset of anchorage-independent growth. Primary cultures of liver cells from R16 rats consistently failed to form cell strains upon subpassage.

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Abbreviations

DEN:

diethylnitrosamine

grc :

growth and reproduction complex

GGT:

gamma-glutamyl transpeptidase

MHC:

major histocompatibility complex

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Authors and Affiliations

  1. Carcinogenesis Research Unit, School of Pathology The University of New South Wales, Kensington, New South Wales, Australia

    Garry J. Smith

  2. Department of Pathology, University of Pittsburgh School of Medicine, 15261, Pittsburgh, Pennsylvania

    Heinz W. Kunz & Thomas J. Gill III

  3. Department of Pathology, University of Texas Medical Branch, Galveston, Texas

    Harold A. Dunsford

Authors
  1. Garry J. Smith
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  2. Heinz W. Kunz
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  3. Harold A. Dunsford
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  4. Thomas J. Gill III
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Smith, G.J., Kunz, H.W., Dunsford, H.A. et al. Histopathology and cell culture characteristics of liver cells from grc − and grc + rats given diethylnitrosamine. Cell Biol Toxicol 6, 205–217 (1990). https://doi.org/10.1007/BF00249594

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  • DOI: https://doi.org/10.1007/BF00249594

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