Summary
Larvae of Ascidia malaca, both before and after hatching, were treated with L-thyroxine solutions. The effect of the thyroid hormone was to induce the onset of metamorphosis and then to cause the rate at which body reorganization occurred to increase. In treated larvae the resorption of the tail occurred only a few hours after hatching, and a beating heart appeared from 10 to 15 h earlier than in the control larvae.
These results are discussed in the context of a probable relationship between the occurrence of a hormonal metamorphic factor and the button cells of the trunk.
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References
Aros B, Virágh SZ (1969) Fine structure of the pharynx and endostyle of an ascidian (Ciona intestinalis). Acta Biol Acad Sci Hung 20:281–297
Bradway W (1936) The experimental alteration of the rate of metamorphosis in the tunicate, Clavelina huntsmani (Van Name). J Exp Zool 72:213–224
Cloney RA (1961) Observations on the mechanism of tail resorption in the ascidians. Am Zool 1:67–87
Cloney RA (1963) The significance of the caudal epidermis in ascidian metamorphosis. Biol Bull 124:241–253
Cloney RA (1978) Ascidian metamorphosis: Review and Analysis. In: Chia FS, Rice M (eds) Settlement and metamorphosis of marine invertebrate larvae. Elsevier, North-Holland Biomedical Press, New York, pp 255–282
Conklin EG (1931) The development of centrifuged eggs of ascidians. J Exp Zool 60:1–119
Derby A (1975) The effect of prolactin and thyroxine on tail resorption in R. pipiens: in vivo and in vitro. J Exp Zool 193:15–20
Dunn AD (1974) Ultrastructural autoradiography and cytochemistry of the iodine-binding cells in the ascidian endostyle. J Exp Zool 188:103–123
Glaser O, Anslow GA (1949) Copper and ascidian metamorphosis. J Exp Zool 111:117–140
Grave C (1935) Metamorphosis of ascidian larvae. Carneg Inst Wash Publ 452:209–292
Grave C (1944) The larva of Styela (Cynthia) partita. Structure, activities and duration of life. J Morphol 75:173–191
Grave C, Nicoll PA (1939) Studies of larval life and metamorphosis in Ascidia nigra and species ofPolyandrocarpa. Carneg Inst Wash Publ 517:1–46
Hirai E (1961) Initiating effect on metamorphosis of vital staining of tadpole larvae of an ascidian, Halocynthia roretzi (v. Drasche). Bull Mar Biol Stat Asamushi 10:189–192
Hirai E (1963) On the duration of swimming larva of an ascidian, Halocynthia roretzi (v. Drasche). Bull Mar Stat Asamushi 11:121–125
Ishikawa M, Numakunai T (1972) Changes in the activity of acid phosphatase and the appearance of metamorphosing potencies of the larvae of the ascidian, Halocynthia roretzi. Dev Growth Diff 14:75–83
Kennedy GR (1966) The distribution and nature of iodine compounds in ascidians. Gen Comp Endocrinol 7:500–511
Lison L (1960) Histochimie et cytochimie animales. Principes et méthodes. Gauthier-Villars, Paris (VIe): Vol II, 398–842
Luna LG (Ed) (1968) Manual of histologie staining methods of the armed forces institute of pathology. McGraw-Hill, Book Co, New York, pp 258
Lynch WF (1961) Extrinsic factors influencing metamorphosis in bryozoan and ascidian larvae. Am Zool 1:59–66
Materazzi G (1967a) Further observations on the embryonic development of ascidians in artificial sea water devoid of sulphate ions. Acta Embryol Morphol Exp 10:101–107
Materazzi G (1967b) Studio sul metabolismo dei mucopolisaccaridi acidi solforati presenti nelle larve di alcune ascidiacee. Boll Soc Ital Biol Sper 43:1919–1922
Materazzi G, Ortolani G (1969) A study of the origin of the cells containing sulfated acid mucopoly-saccharides in the cephalic portion of the larvae of Phallusia mamillata and Ascidia malaca. Dev Biol 20:378–385
Numakunai T (1967) Tail shortening of the larvae of an ascidian, Halocynthia roretzi (v.Drasche), separated at various positions after stimulation to metamorphose. Bull Mar Biol Stat Asamushi 13:7–11
Ortolani G, Patricolo E (1972) Further data on some particular cells of the cephalenteron of the larvae of some ascidians. Arch Biol (Liège) 83:1–9
Reverberi G (1971) Osservazioni sulle cellule “a perossidasi” di alcune larve di Ascidie al microscopio elettronico. Acc Naz Lincei 50:795–802
Reverberi G, Ortolani G, Fernandez M (1969) Su alcune singolari cellule “a perossidasi” delie larve di Ascidie. Acc Naz Lincei 47:375–384
Thorndyke MC (1978) Evidence for “a mammalian” thyroglobulin in endostyle of the ascidian Styela clava. Nature 271:61–62
Thorpe A, Thorndyke MC (1975) The endostyle in relation to iodine binding. In: Barrington JW, Jefferies RPS (eds) Protochordates. Symp Zool Soc Lond 36:159–177, Academic Press, London
Thorpe A, Thorndyke MC, Barrington JW (1972) Ultrastructural and histochemical features of the endostyle of the ascidian Ciona intestinalis with special reference to the distribution of bound iodine. Gen Comp Endocrinol 19:559–571
Weber R (1967) Biochemistry of amphibian metamorphosis. In: Weber R (ed) The biochemistry of animal development. Academic Press, New York, Vol II, 227–301
Weiss P (1928) Experimentelle Untersuchungen über die Metamorphose der Ascidien. Biol Zentr 48:69–79
Whittaker JR (1964) Copper as a factor in the onset of ascidian metamorphosis. Nature 202:1024–1025
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Patricolo, E., Ortolani, G. & Cascio, A. The effect of L-thyroxine on the metamorphosis of Ascidia malaca . Cell Tissue Res. 214, 289–301 (1981). https://doi.org/10.1007/BF00249213
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DOI: https://doi.org/10.1007/BF00249213