Abstract
Methanosarcina thermophila TM-1 produced hydrogen during growth on acetate, maintaining a concentration of approx. 0.3 μM in the culture, corresponding to a hydrogen partial pressure of approx. 40 Pa. Increasing the partial pressure of hydrogen to 250 Pa and more led to a gradually increasing inhibition of acetate metabolism. No growth was observed when the gas phase contained 2000 Pa or more and acetate metabolism did not occur even after prolonged incubation (more than 2 weeks). M. thermophila was capable of limited consumption of hydrogen. Consumption of low concentrations of hydrogen proceeded simultaneously with acetate utilization. The affinity for hydrogen (K m=5 μM) was within the range normally found for hydrogenutilizing methanogens, while the corresponding V max (1.2 μmol hydrogen consumed mg-1 · cells h-1) was orders of magnitude lower.
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References
Ahring BK, Westermann P (1987) Kinetics of butyrate, acetate, and hydrogen metabolism in a thermophilic anaerobic, butyratedegrading triculture. Appl Environ Microbiol 53: 434–439
Ahring BK, Westermann P (1988) Product inhibition of butyrate metabolism by acetate and hydrogen in a thermophilic coculture. Appl Environ Microbiol 54: 2393–2397
Ahring BK, Alatriste-Mondragon F, Westermann P, Mah RA (1991) Effects of cations on Methanosarcina thermophila TM-1 growing on moderate concentrations of acetate: production of single cells. Appl Microbiol Biotechnol 35: 686–689
Blaut M, Gottschalk G (1982) Effect of trimethylamine on acetate utilization by Methanosarcina barkeri. Arch Microbiol 133: 230–235
Boone DR, Menaia JAGF, Boone JE, Mah RA (1987) Effects of hydrogen pressure during growth and effects of pregrowth with hydrogen on acetate degradation by Methanosarcina species. Appl Environ Microbiol 53: 83–87
Boone DR, Mah RA (1987) Effects of molecular hydrogen on acetate degradation by Methanosarcina barkeri 227. In: Cardoso Duarte, Archer LJ, Bull AT, Holst G (eds) Perspectives in biotechnology. Plenum Publishing Corporation, New York, pp 111–121
Bott M, Eikmanns B, Thauer RK (1986) Coupling of carbon monoxide oxidation to CO2 and H2 with phosphorylation of ADP in acetate grown Methanosarcina barkeri. Eur J Biochem 159: 393–398
Ferguson TJ, Mah RA (1983) Effects of H2-CO2 on methanogenesis from acetate or methanol in Methanosarcina spp. Appl Environ Microbiol 46: 348–355
Fischer R, Thauer RK (1990) Methanogenesis from acetate in cell extracts of Methanosarcina barkeri: isotope exchange between CO2 and the carbonyl group of acetyl-CoA, and the role of H2. Arch Microbiol 153: 156–162
Lovley DR, Ferry JG (1985) Production and consumption of H2 during growth of Methanosarcina spp. on acetate. Appl Environ Microbiol 49: 247–249
Macario A, Earle JFK, Chynoweth DP, Conway De Macario E (1989) Distinctive patterns of methanogenic flora determined with antibody probes in anaerobic digestors of different characteristics operated under controlled conditions. System Appl Microbiol 12: 216–222
Phelps TJ, Conrad R, Zeikus JG (1985) Sulfate-dependent interspecies H2 transfer between Methanosarcina barkeri and Desulfovibrio vulgaris during cocultures metabolism of acetate or methanol. Appl Environ Microbiol 50: 589–594
Scherer P, Sahm H (1981) Influence of sulphur containing compounds on the growth of Methanosarcina barkeri in a defined medium. Eur J Appl Microbiol Biotechnol 12: 28–35
Segel IH (1968) Biochemical calculations. John Wiley & Sons, New York
Switzenbaum MS, Giraldo-Gomez E, Hickey RF (1990) Monitoring of the anaerobic methane fermentation process. Enzyme Microbiol Technol 12: 722–730
Westermann P, Ahring BK, Mah RA (1989) Temperature compensation in Methanosarcina barkeri by modulation of hydrogen and acetate affinity. Appl Environ Microbiol 55: 1262–1266
Worakit SD, Boone DR, Mah RA, Abdel-Samie M-E, El-Halwagi MM (1986) Methanobacterium alcaliphilum sp. nov., and H2-utilizing methanogen that grows at high pH values. Int J Syst Bacteriol 36: 380–382
Zehnder AJB, Ingvorsen K, Marti T (1982) Microbiology of methane bacteria. In: Hughes DE (ed) Anaerobic digestion. Elsevier/North Holland, Amsterdam, pp 45–64
Zinder SH, Mah RA (1979) Isolation and characterization of a thermophilic strain of Methanosarcina unable to use H2-CO2 for methanogenesis. Appl Environ Microbiol 38: 996–1008
Zinder SH, Sowers KR, Ferry JG (1985) Methanosarcina thermophila sp. nov., a thermophilic acetotrophic methane-producing bacterium. Int J Syst Bacteriol 35: 522–523
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Ahring, B.K., Westermann, P. & Mah, R.A. Hydrogen inhibition of acetate metabolism and kinetics of hydrogen consumption by Methanosarcina thermophila TM-1. Arch. Microbiol. 157, 38–42 (1991). https://doi.org/10.1007/BF00245332
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DOI: https://doi.org/10.1007/BF00245332