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The lipopolysaccharides of Rhodospirillum rubrum, Rhodospirillum molischianum, and Rhodopila globiformis

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Abstract

The cell wall lipopolysaccharides from three phototrophic species of the alpha1-group of Proteobacteria, Rhodospirillum rubrum, Rhodospirillum molischianum, and Rhodopila globiformis were isolated and chemically characterized. Sodium deoxycholate polyacrylamide gel electrophoresis patterns revealed that the lipopolysaccharides of all three species possess O-chains. They are composed of repeating units only in R. molischianum and R. globiformis. The presence of l-glycero-d-mannoheptose and 2-keto-3-deoxyoctonate indicated core structures in all three lipopolysaccharides. Glucosamine was found as backbone amino sugar in lipid A of R. molischianum and R. rubrum, while R. globiformis has 2,3-diaminoglucose as backbone amino sugar. The latter species also differed from the two former ones in its content of hydroxy fatty acids (3-OH-14:0, 3-OH-16:0 in R. rubrum and R. molischianum and 3-OH-14:0, 3-OH-18:0 and 3-OH-19:0 (possibly iso- or anteisobranched) in R. globiformis).

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Abbreviations

DOC-PAGE:

sodium deoxycholate polyacrylamide gel electrophoresis

GC/MS:

combined gas-liquid chromatography/mass spectrometry

KDO:

2-keto-3-deoxyoctonate

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Authors and Affiliations

  1. Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-7800, Freiburg i. Br., Germany

    Klaus Pietsch & Hubert Mayer

  2. Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Schänzlestrasse 1, D-7800, Freiburg i. Br., Germany

    Jürgen Weckesser

  3. Fachbereich Biologie, AG Geomikrobiologie, Universität Oldenburg, Postfach 2503, D-2900, Oldenburg, Germany

    Ulrich Fischer

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  1. Klaus Pietsch
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  2. Jürgen Weckesser
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  3. Ulrich Fischer
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  4. Hubert Mayer
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Pietsch, K., Weckesser, J., Fischer, U. et al. The lipopolysaccharides of Rhodospirillum rubrum, Rhodospirillum molischianum, and Rhodopila globiformis . Arch. Microbiol. 154, 433–437 (1990). https://doi.org/10.1007/BF00245223

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