Abstract
The halotolerant, facultatively chemolithoautotrophic, gramnegative sulphur-oxidizing organism isolated from the gills of Thyasira flexuosa is shown to exhibit pleomorphism consistent with its being a member of the genus Thiomicrospira and not a Thiobacillus as originally classified. Its distinctive morphology, DNA base composition, and similarity of 16S rRNA sequences to that of Thiomicrospira sp. strain L-12 (which differs only in detail from the type species Thiomicrospira pelophila), lead us to reclassify this strain as Thiomicrospira thyasirae DSM5322.
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Distel DL, Wood AP (1992) Characterization of the gill symbiont of Thyasira flexuosa (Thyasiridae: Bivalvia) by use of polymerase chain reaction and 16S rRNA sequence analysis. J Bacteriol 174: 6317–6320
Harrison AP (1989) Genus Acidiphilium Harrison. In: Staley JT, Bryant MP, Pfennig N, Holt JG (eds.) Bergey's manual of systematic bacteriology, vol. 3. Williams and Wilkins, Baltimore, pp 1863–1868
Imhoff JF (1982) The family Ectothiorhodospiraceae. In: Starr MP, Stolp H Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes, 1st edn. Berlin Heidelberg New York, Springer pp 126–128
Imhoff JF, Trüper HG (1977) Ectothiorhodospira halochloris sp. nov., a new extremely halophilic phototrophic bacterium containing bacteriochlorophyll b. Arch Microbiol 114: 115–121
Jannasch HW, Wirsen CO, Nelson DC, Robertson LA (1985) Thiomicrospira crunogena sp nov, a colorless, sulfur-oxidizing bacterium from a deepsea hydrothermal vent. Int J Syst Bacteriol 35: 422–424
Kelly DP, Harrison AP (1989) Genus Thiobacillus Beijerinck. In: Staley JT Bryant MP, Pfennig N, Holt JG (eds) Bergey's manual of systematic bacteriology, vol 3. Williams and Wilkins, Baltimore, pp 1842–1858
Kuenen JG, Veldkamp H (1972) Thiomicrospira pelophila, gen. n., sp. n., a new obligately chemolithotrophic colourless sulfur bacterium. Antonie van Leeuwenhoek 38: 241–256
Kuenen JG, Tuovinen OH (1982) The genera Thiobacillus and Thiomicrospira. In: Starr MP, Stolp H, Trüper HP, Balows A, Schlegel HG (eds) The prokaryotes, 1 st edn. Berlin Heidelberg New York, Springer, pp. 1023–1036
Kuenen JG, Robertson LA (1989a) Genus Thiomicrospira Kuenen and Veldkamp. In: Staley JT, Bryant MP, Pfennig N, Holt JG (eds) Bergey's manual of systematic bacteriology, vol. 3. Williams and Wilkins, Baltimore, pp 1858–1861
Kuenen JG, Robertson LA (1989b) Genus Thiosphaera Kuenen and Robertson. In: Staley JT, Bryant MP, Pfennig N, Holt JG (eds) Bergey's manual of systematic bacteriology, vol. 3. Williams and Wilkins, Baltimore, pp. 1861–1862
Kuenen JG, Robertson LA Tuovinen OH (1991) The genera Thiobacillus, Thiomicrospira, and Thiosphaera. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H (eds) The prokaryotes, 2nd edn. Springer, New York Berlin Heidelberg, pp 2638–2657
Lane DJ, Harrison AP, Stahl D, Pace B, Giovannoni SJ, Olsen GJ, Pace NR (1992) Evolutionary relationships among sulfur- and iron-oxidizing eubacteria. J Bacteriol 174: 269–278
Robertson LA, Kuenen JG (1991) The colorless sulfur bacteria. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H (eds) The prokaryotes, 2nd edn. Springer, New York Berlin Heidelberg, pp 385–413
Ruby EG, Wirsen CO, Jannasch HW (1981) Chemolithotrophic sulfur-oxidizing bacteria from the Galapagos Rift hydrothermal vents. Appl Env Microbiol 42: 317–324
Stahl DA, Lane DJ, Olsen GJ, Heller DJ, Schmidt TM, Pace NR (1987) Phylogenetic analysis of certain sulfide-oxidizing and related morphologically conspicuous bacteria by 5S ribosomal ribonucleic acid sequences. Int J Syst Bacteriol 37: 116–122
Timmer-ten Hoor A (1975) A new type of thiosulphate oxidizing nitrate reducing microorganism. Thiomicrospira denitrificans sp. nov. Netherlands J Sea Res 9: 344–350
Wood AP, Kelly DP (1989) Isolation and physiological characterisation of Thiobacillus thyasiris sp. nov., a novel marine facultative autotroph and the putative symbiont of Thyasira flexuosa. Arch Microbiol 152: 160–166
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Wood, A.P., Kelly, D.P. Reclassification of Thiobacillus thyasiris as Thiomicrospira thyasirae comb. nov., an organism exhibiting pleomorphism in response to environmental conditions. Arch. Microbiol. 159, 45–47 (1993). https://doi.org/10.1007/BF00244262
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DOI: https://doi.org/10.1007/BF00244262