Abstract
Phosphate-independent nucleoside deoxyribosyltransferase activity was detected in Aerococcus viridans, Leuconostoc mesenteroides, two species of Pediococcus, eight obligately homofermentative and three obligately heterofermentative lactobacilli. In cellfree extracts of one strain of Lactococcus, one strain of Streptococcus and three strains of facultatively heterofermentative lactobacilli no phosphate-independent nucleoside deoxyribosyltransferase could be detected and inosine nucleoside phosphorylase was the only enzyme catalysing pentosyl transfer to purines. A classification based on the presence or absence of these enzymes separates Streptococcus and Lactococcus from the remaining genera which is in agreement with studies using anti-aldolase sera and 16S rRNA oligonucleotide catalogues.
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Abbreviations
- NCIMB:
-
National Collection of Industrial and Marine bacteria
- NCDO:
-
National Collection of Dairy organisms
- MRS:
-
deMan Rogosa Sharpe
- Pipes:
-
Piperazine-N,N′-bis[2-ethanesulphonic acid]
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Chawdhri, R.F., Hutchinson, D.W. & Richards, A.O. Nucleoside deoxyribosyltransferase and inosine phosphorylase activity in lactic acid bacteria. Arch. Microbiol. 155, 409–411 (1991). https://doi.org/10.1007/BF00243463
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DOI: https://doi.org/10.1007/BF00243463