Summary
The specificity of d-[3H]-aspartate as a retrograde marker was investigated following large injections of this tritiated amino acid in the monkey cerebellar cortex. Retrogradely labeled neurons were found exclusively in the inferior olive, a fact which is consistent with previous studies in the rat. These results show that d-[3H]-aspartate is a selective tracer that might also be useful to delineate excitatory pathways using glutamate and/or aspartate as neuro-transmitter(s) in the monkey brain.
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References
Baughman RW, Gilbert CD (1981) Aspartate and glutamate as possible neurotransmitters in the visual cortex. J Neurosci 1: 427–439
Brodal P, Brodal A (1981) The olivocerebellar projection in the monkey. Experimental studies with the method of retrograde tracing of horseradish peroxidase. J Comp Neurol 201: 375–393
Brodal A, Kawamura K (1980) Olivocerebellar projection: a review. Adv Anat 64: 1–140
Cuénod M, Bagnoli P, Beaudet A, Rustioni A, Wiklund L, Streit P (1982) Transmitter-specific retrograde labeling of neurons. In: Chan-Palay V, Palay SL (eds) Cytochemical methods in neuroanatomy. Alan R Liss, New York, pp 17–44
Cuénod M, Streit P (1983) Neuronal tracing using retrograde migration of labeled transmitter-related compounds. In: Björklund A, Hökfelt T (eds) Handbook of chemical neuroanatomy, Vol. 1. Methods in chemical neuroanatomy. Elsevier, Amsterdam, pp 365–397
Gould BB (1980) Organization of afferents from the brain stem nuclei to the cerebellar cortex in the cat. Adv Anat Embryol Cell Biol 62: 1–90
Ito M (1984) The cerebellum and neural control. Raven Press, New York, pp 133–283
Kotchabhakdi N, Walberg F, Brodal A (1978) The olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. VII. The projection to lobulus simplex, crus I and II. J Comp Neurol 182: 293–314
Künzle H, Wiklund L (1982) Identification and distribution of neurons presumed to give rise to cerebellar climbing fibers in turtle. A retrograde axonal flow study using radioactive d-aspartate as a marker. Brain Res 252: 146–150
Matute C, Streit P (1985) Selective retrograde labeling with d-[3H]-aspartate in afferents to the mammalian superior colliculus. J Comp Neurol 241: 34–49
Streit P (1984) Glutamate and aspartate as transmitter candidates for systems of the cerebral cortex. In: Peters A, Jones EG (eds) Cerebral cortex, Vol 2. Plenum Press, New York, pp 119–143
Toggenburger G, Wiklund L, Henke H, Cuénod M (1983) Release of endogenous and accumulated exogenous amino acids from slices of normal and climbing fibre-deprived rat cerebellar slices. J Neurochem 41: 1606–1613
Wannier TMJ, Töltl M, Hepp-Reymond MC (1986) Neuronal activity in the postcentral cortex related to force regulation during a precision grip task. Brain Res 382: 427–432
Wiklund L, Toggenburger G, Cuénod M (1982) Aspartate: possible neurotransmitter in cerebellar climbing fibers. Science 216: 78–79
Wiklund L, Toggenburger G, Cuénod M (1984) Selective retrograde labelling of the rat olivocerebellar climbing fiber system with d-[3H]-aspartate. Neuroscience 13: 441–468
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Matute, C., Wiklund, L., Streit, P. et al. Selective retrograde labeling with D-[3H]-aspartate in the monkey olivocerebellar projection. Exp Brain Res 66, 445–447 (1987). https://doi.org/10.1007/BF00243320
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DOI: https://doi.org/10.1007/BF00243320