Skip to main content
SpringerLink
Log in

Aliphatic 1H and 13C resonance assignments for the 26-10 antibody VL domain derived from heteronuclear multidimensional NMR spectroscopy

  • Research Papers
  • Published:
Journal of Biomolecular NMR Aims and scope Submit manuscript

Summary

Extensive 1H and 13C assignments have been obtained for the aliphatic resonances of a uniformly 13C-and 15N-labeled recombinant VL domain from the anti-digoxin antibody 26-10. Four-dimensional triple resonance NMR data acquired with the HNCAHA and HN(CO)CAHA pulse sequences [Kay et al. (1992) J. Magn. Reson., 98, 443–450] afforded assignments for the backbone HN, N, Hα and Cα resonances. These data confirm and extend HN, N and Hα assignments derived previously from three-dimensional 1H-15N NMR studies of uniformly 15N-labeled VL domain [Constantine et al. (1992), Biochemistry, 31, 5033–5043]. The identified Hα and Cα resonances provided a starting point for assigning the side-chain aliphatic 1H and 13C resonances using three-dimensional HCCH-COSY and HCCH-TOCSY experiments [Clore et al. (1990), Biochemistry, 29, 8172–8184]. The Cα and Cβ chemical shifts are correlated with the VL domain secondary structure. The extensive set of side-chain assignments obtained will allow a detailed comparison to be made between the solution structure of the isolated VL domain and the X-ray structure of the VL domain within the 26–10 Fab.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anthony, J., Near, R., Wong, S., Iida, E., Ernst, E., Wittekind, M., Haber, E. and Ng, S.-C. (1992) Mol. Immunol., 29, 1237–1247.

    Google Scholar 

  • Bax, A., Clore, G.M., Driscoll, P.C., Gronenborn, A.M., Ikura, M. and Kay, L.E. (1990a) J. Magn. Reson., 87, 620–627.

    Google Scholar 

  • Bax, A., Clore, G.M. and Gronenborn, A.M. (1990b) J. Magn. Reson., 88, 425–431.

    Google Scholar 

  • Chothia, C., Novotny, J., Bruccoleri, R. and Karplus, M. (1985) J. Mol. Biol., 186, 651–663.

    Google Scholar 

  • Clore, G.M., Bax, A., Driscoll, P.C., Wingfield, P.T. and Gronenborn, A.M. (1990) Biochemistry, 29, 8172–8184.

    Google Scholar 

  • Constantine, K.L., Goldfarb, V., Wittekind, M., Anthony, J., Ng, S.-C. and Mueller, L. (1992a) Biochemistry, 31, 5033–5043.

    Google Scholar 

  • Constantine, K.L., Friedrichs, M.S., Goldfarb, V., Jeffrey, P.D., Sheriff, S. and Mueller, L. (1992b) Proteins Struct. Funct. Genet., in press.

  • Fairbrother, W.J., Palmer, A.G., Rance, M., Reizer, J., Saier Jr., M.H. and Wright, P.E. (1992) Biochemistry, 31, 4413–4425.

    Google Scholar 

  • Grzesiek, S. and Bax, A. (1992) J. Magn. Reson., 99, 201–207.

    Google Scholar 

  • Herron, J.N., He, X.M., Mason, M.L., Voss Jr., E.W. and Edmundson, A.B. (1989) Proteins Struct. Funct. Genet., 5, 271–280.

    Google Scholar 

  • Herron, J.N., He, X.M., Ballard, D.W., Blier, P.R., Pace, P.E., Bothwell, A.L.M., Voss Jr., E.W. and Edmundson, A.B. (1991) Proteins Struct. Funct. Genet., 11, 159–175.

    Google Scholar 

  • Kay, L.E., Ikura, M., Tschudin, R. and Bax, A. (1990) J. Magn. Reson., 89, 496–514.

    Google Scholar 

  • Kay, L.E., Wittekind, M., McCoy, M.A., Friedrichs, M.S. and Mueller, L. (1992) J. Magn. Reson., 98, 443–450.

    Google Scholar 

  • Marion, D., Ikura, M. and Bax, A. (1989a) J. Magn. Reson., 84, 425–428.

    Google Scholar 

  • Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989b) J. Magn. Reson., 85, 393–399.

    Google Scholar 

  • McCoy, M.A. and Mueller, L. (1992) J. Am. Chem. Soc., 114, 2108.

    Google Scholar 

  • McManus, S. and Riechman, L. (1991) Biochemistry, 30, 5851–5857.

    Google Scholar 

  • Mueller, L. and Ernst, R.R. (1979) Mol. Phys., 38, 963–992.

    Google Scholar 

  • Powers, R., Garrett, D.S., March, C.J., Frieden, E.A., Gronenborn, A.M. and Clore, G.M. (1992) Biochemistry, 31, 4334–4346.

    Google Scholar 

  • Schildbach, J.F., Panka, D.J., Parks, D.R., Jager, G.C., Herzenberg, L.A., Mudgett-Hunter, M., Haber, E. and Margolies, M.N. (1991) J. Biol. Chem., 266, 4640–4647.

    Google Scholar 

  • Shaka, A.J., Keeler, J., Frenkiel, T. and Freeman, R. (1983) J. Magn. Reson., 52, 355.

    Google Scholar 

  • Shaka, A.J., Lee, C.J. and Pines, A. (1988) J. Magn. Reson., 77, 274–293.

    Google Scholar 

  • Smith, T.W., Haber, E., Yeatman, L. and Butler Jr., V.P. (1976) N. Engl. J. Med., 294, 8797.

    Google Scholar 

  • Spera, S. and Bax, A. (1991) J. Am. Chem. Soc., 113, 5490–5492.

    Google Scholar 

  • States, D.J., Haberkorn, R.A. and Ruben, D.J. (1982) J. Magn. Reson., 48, 286–292.

    Google Scholar 

  • Takahashi, H., Odaka, A., Kawaminami, S., Matsunga, C., Kato, K., Shimada, I. and Arata, Y. (1991) Biochemistry, 30, 6611–6619.

    Google Scholar 

  • Takahashi, H., Suzuki, E., Shimada, I. and Arata, Y. (1992) Biochemistry, 31, 2464–2468.

    Google Scholar 

  • Wishart, D.S., Sykes, B.D. and Richards, F.M. (1991) J. Mol. Biol., 222, 311–333.

    Google Scholar 

  • Wittekind, M., Gorlach, M., Friedrichs, M., Dreyfuss, G. and Mueller, L. (1992) Biochemistry, 31, 6254–6265.

    Google Scholar 

  • Wright, P.E., Dyson, H.J., Lerner, R.A., Riechman, L. and Tsang, P. (1990) Biochem. Pharmacol., 40, 83–88.

    Google Scholar 

  • Zhu, G. and Bax, A. (1992) J. Magn. Reson., 90, 405.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bristol-Myers Squibb Pharmaceutical Research Institute, P.O. Box 4000, 08543, Princeton, NJ, U.S.A.

    Keith L. Constantine, Valentina Goldfarb, Michael Wittekind, Mark S. Friedrichs, James Anthony, Shi-Chung Ng & Luciano Mueller

Authors
  1. Keith L. Constantine
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Valentina Goldfarb
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael Wittekind
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark S. Friedrichs
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. James Anthony
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shi-Chung Ng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Luciano Mueller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Constantine, K.L., Goldfarb, V., Wittekind, M. et al. Aliphatic 1H and 13C resonance assignments for the 26-10 antibody VL domain derived from heteronuclear multidimensional NMR spectroscopy. J Biomol NMR 3, 41–54 (1993). https://doi.org/10.1007/BF00242474

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00242474

Keywords

Search

Navigation