Abstract
Production of berberine could be induced by adding 6-benzylaminopurine (BAP) to Thalictrum minus cells, cultured in suspension in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), early in the growth cycle. In the presence of BAP, the precursor, L-tyrosine, was rapidly converted into berberine which was then released into the medium, whereas substantial amounts of the intermediates, tyramine and dopamine, accumulated in non-berberine-producing cells grown in the same 2,4-D-containing medium without BAP. These results suggest that BAP activated enzymatic reactions subsequent to the formation of the amines in the biosynthesis of berberine.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BAP:
-
6-benzylaminopurine
- NAA:
-
1-naphthaleneacetic acid
- IAP:
-
6-isopentenylaminopurine
- LS medium:
-
Linsmaier-Skoog medium
- “Growth medium”:
-
LS medium containing 10-6 M 2,4-D
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Hara, M., Kitamura, T., Fukui, H. et al. Induction of berberine biosynthesis by cytokinins in Thalictrum minus cell suspension cultures. Plant Cell Reports 12, 70–73 (1993). https://doi.org/10.1007/BF00241937
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DOI: https://doi.org/10.1007/BF00241937