Abstract
The expression of the genes for two types of myrosinase (EC 3.2.3.1), designated MA and MB, during embryo and seedling development was investigated in Sinapis alba L. by in-situ and RNA slot-blot analyses. The expression of MA and MB genes followed similar temporal profiles during embryogenesis, but MB mRNA was present in considerably higher amounts than MA mRNA. In the embryo, both MA and MB genes are activated in cotyledons and axis. The MB genes are preferentially expressed in the cotyledons whereas MA genes are preferentially expressed in the axis. In the developing seedling, MA mRNA was not present in the organs investigated. By contrast, MB mRNA was found in appreciable amounts in hypocotyls, cotyledons and developing leaves. The MB genes seem to be activated preferentially in tissues undergoing rapid cell division and — or cell expansion.
Similar content being viewed by others
Abbreviations
- DAP:
-
days after pollination
- MA, MB:
-
A type, B type myrosinases in Sinapis alba
References
Björkman, R., Janson, J.-C. (1972) Studies on myrosinase. I. Purification and characterization of a myrosinase from white mustard seed (Sinapis alba L.). Biochim. Biophys. Acta. 276, 508–518
Bones, A. (1990) Distribution of β-thioglucosidase activity in intact plants, cell and cultures and regenerate plants of Brassica napus L.J. Exp. Bot. 41, 737–744
Bones, A., Iversen, T.-H. (1985) Myrosin cells and myrosinase. Isr. J. Bot. 34, 351–376
Cox, K.H., Goldberg, R.B. (1988) Analysis of plant gene expression. In: Plant molecular biology: A practical approach, pp. 1–34, Shaw, C.H., ed. IRL Press, Oxford
Fahn, A. (1979) Secretory tissues in plants. Academic Press, London
Falk, A., Xue, J., Lenman, M., Rask, L. (1992) Sequence of a cDNA clone encoding the enzyme myrosinase and expression of myrosinase in different tissues of Brassica napus. Plant Sci. 83, 181–186
Höglund, A.-S., Lenman, M., Falk, A., Rask, L. (1991) Distribution of myrosinase in rapeseed tissues. Plant Physiol. 95, 213–221
Höglund, A.-S., Lenman, M., Rask, L. (1992) Myrosinase is localized to the interior of myrosin grains and is not associated to surrounding tonoplast membrane. Plant Sci. 85, 165–170
James, C.D., Rossiter, T.J. (1991) Development and characteristics of myrosinase in Brassica napus during early seeding growth. Physiol. Plant. 82, 162–170
Lenman, M., Falk, A., Xue, J., Rask, L. (1993) Characterization of a Brassica napus myrosinase pseudogene: myrosinases are members of the BGA family of β-glycosidases. Plant Mol. Biol. 21, 463–474
Louda, S.M., Rodman, J.E. (1983) Ecological patterns in the glucosinolate content of a native mustard, Cardamine cordifolia, in the Rocky Mountains. J. Chem. Ecol. 9, 397–422
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–4197
Pihakaski, K., Pihakaski, S. (1978) Myrosinase in Brassicaceae (Cruciferae). J. Exp. Bot. 29, 335–345
Selden, R.F. (1987–1989) Analysis of RNA by northern hybridization. In: Current protocols in molecular biology, pp. 4.91–4.94, Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K., eds. Wiley-Interscience, New York
Srivastava, V.K., Hill, D.C. (1974) Glucosinolate hydrolytic products given by Sinapis alba and Brassica napus thioglucosidase. Phytochemistry 13, 1043–1046
Thangstad, O.P., Iversen, T.-H., Slupphaug, G., Bones, A. (1990) Immunocytochemical localization of myrosinase in Brassica napus. Planta 180, 245–248
Thangstad, O.P., Evjen, K., Bones, A. (1991) Immunogold-EM localization of myrosinase in Brassicaceae. Protoplasma 161, 85–93
Uppström, B. (1983) Glucosinolate pattern in different growth stages of high and low glucosinolate varieties of Brassica napus. J. Swedish Seed Assoc. 4, 331–336
Xue, J., Lenman, M., Falk, A., Rask, L. (1992) The glucosinolatedegrading enzyme myrosinase in Brassicaceae is encoded by a gene family. Plant Mol. Biol. 18, 387–398
Author information
Authors and Affiliations
Additional information
Anna-Stina Höglund (Uppsala Genetic Center) is gratefully acknowledged for valuable discussion, Anders Gobl (Department of Immunology, Uppsala University) for kindly advice with the labeling of probes and Qingzhu Zhai (Department of Pharmaceutical Biosciences, Uppsala University) for help with seed harvest. This work was supported by grants from the Swedish Research Council for Forestry and Agriculture.
Rights and permissions
About this article
Cite this article
Xue, J., Pihlgren, U. & Rask, L. Temporal, cell-specific, and tissue-preferential expression of myrosinase genes during embryo and seedling development in Sinapis alba . Planta 191, 95–101 (1993). https://doi.org/10.1007/BF00240900
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00240900