Abstract
The effect of microinjected calcium-loaded 1,2-bis(2-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid (CaBAPTA) on cell-to-cell diffusion of carboxyfluorescein (CF) was examined in staminal hairs of S. purpurea Boom. The CaBAPTA was microinjected into the cytoplasm of the staminal hairs either with CF or prior to a subsequent microinjection of CF. The cell-to-cell diffusion of CF along the hair was monitored using enhanced-fluorescence video microscopy. Cytoplasmic streaming stopped in cells treated with CaBAPTA, indicating that intracellular Ca2+ had increased. Cell-to-cell diffusion of CF was blocked in cells treated with Ca-BAPTA. An inhibition of cytoplasmic streaming and cell-to-cell diffusion was observed in the cells adjoining the CaBAPTA-microinjected cell, indicating that the Ca-BAPTA appeared to pass through plasmodesmata. While cytoplasmic streaming resumed 5–10 min after CaBAPTA treatment, cell-to-cell diffusion did not resume until 30–120 min later. These data support an involvement of calcium in the regulation of cell-to-cell communication in plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BAPTA:
-
1,2-bis(2-aminophenoxy)ethane-N,N,N′, N′-tetraacetic acid
- CF:
-
carboxyfluorescein
Reference
Baron-Epel, O., Hernandez, D., Jiang, L-W., Meiners, S. Schindler, M. (1988) Dynamic continuity of cytoplasmic and membrane compartments between plant cells. J. Cell Biol. 106, 715–721
Berridge, M.J. (1984) Inositol trisphosphate and diacylglycerol as second messengers. Biochem. J. 220, 345–360
Boss, W.F., Morré, D.J., ed. (1989) Second messengers in plant growth and development. Liss, New York
Carr, D.J. (1976) Plasmodesmata in growth and development. In: Intercellular communication in plants: Studies on plasmodesmata, pp. 243–289, Gunning, B.E.S., Robards, A.W., eds. Springer, New York
Dorée, M., Picard, A. (1980) Release of Ca2+ from intracellular pools stops cytoplasmic streaming in Tradescantia staminal hairs. Experientia 36, 1291–1292
Erwee, M.G., Goodwin, P.B. (1983) Characterization of the Egeria densa Planch. leaf symplast. Inhibition of the intracellular movement of fluorescent probes by group II ions. Planta 158, 124–130
Gilroy, S., Blowers, D.P., Trewavas, A.J. (1987) Calcium: a regulation system emerges in plant cells. Development 100, 181–184
Gunning B.E.S., Overall, R.L. (1983) Plasmodesmata and cell-to-cell transport in plants. BioScience 4, 260–265
Hepler, P.K., Callaham, D.A. (1987) Free calcium increases during anaphase in stamen hair cells of Tradescantia. J. Cell Biol. 105, 2137–2143
Hepler, P.K., Palevitz, B.A. (1974) Microtubules and microfilaments. Annu. Rev. Plant Physiol. 25, 309–362
Kamiya, N. (1986) Cytoplasmic streaming in giant algal cells: A historical survey of experimental approaches. Bot. Mag. Tokyo 99, 441–467
López-Sáez, J.F., Giménez-Martin, G., Risueño, M.C. (1965) Fine structure of the plasmodesm. Protoplasma 61, 81–84
Meiners, S., Schindler, M. (1987) The immunological evidence for gap junction polypeptides in plant cells. J. Biol. Chem. 262, 951–953
Mimura, T., Tazawa, M. (1983) Effect of intracellular calcium on membrane potential and membrane resistance in tonoplast free cells of Nitellopsis obtusa. Protoplasma 118, 49–55
Olesen, P. (1979) The neck constriction in plasmodesmata: Evidence for a peripheral sphincter-like structure revealed by fixation with tannic acid. Planta 144, 349–358
Overall, R.L., Wolfe, J.G., Gunning, B.E.S. (1982) Intercellular communication in Azolla roots. I. Ultrastructure of plasmodesmata. Protoplasma 111, 134–150
Pethig, R., Kuhn, M., Payne, R., Alder, E., Chen, T.-H., Jaffe, L.F. (1989) On the dissociation constants of BAPTA-type calcium buffers. Cell Calcium 10, 491–498
Poovaiah, B.W., Reddy, A.S.N., McFadden, J.J. (1987) Calcium messenger system: role of protein phosphorylation and inositol bisphospholipids. Physiol. Plant. 69, 569–573
Robards, A.W., Clarkson, D.T. (1984) Effect of chilling temperatures on root cell membranes as viewed by freeze-fracture electron microscopy. Protoplasma 122, 75–85
Rose, B., Loewenstein, W.R. (1975) Permeability of cell junction depends on local cytoplasmic calcium activity. Nature 254, 250–251
Speksnijder, J.E., Miller, A.L., Weisenseel, M.H., Chen, T.-H., Jaffe, L.F. (1989) Calcium buffer injections block fucoid egg development by facilitating calcium diffusion. Proc. Natl. Acad. Sci. USA 86, 6607–6611
Tsien, R.Y. (1980) New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structure. Biochemistry 19, 2396–2404
Tucker E.B. (1982) Translocation in the staminal hairs of Setcreasea purpurea. I. A study of cell ultrastructure and cell-to-cell passage of molecular probes. Protoplasma 113, 193–201
Tucker, E.B. (1987) Cytoplasmic streaming does not drive intercellular passage in staminal hairs of S. purpurea. Protoplasma 137, 140–144
Tucker, E.B. (1988) Inositol bisphosphate and inositol trisphosphate inhibit cell-to-cell passage of carboxyfluorescein in staminal hairs of Setcreasea purpurea. Planta 174, 358–363
Tucker, E.B. (1990) Inositol phosphates and diacylglycerols inhibit cell-to-cell transport. In: Inositol metabolism in plants, Morrè, J.D., Boss, W.F., Loewus, F., eds. (Plant Biol. Ser.), in press. Liss, New York
Tucker, E.B., Allen, N.S. (1986) Intracellular particle motions (cytoplasmic streaming) in staminal hairs of Setcreasea purpurea: Effect of azide and low temperature. Cell Motil. Cytoskel. 6, 305–313
Tucker, J.E., Mauzerall, D., Tucker, E.B. (1989) Symplastic transport of carboxyfluorescein in staminal hairs of Setcreasea purpurea is diffusive and included loss to the vacuole. Plant Physiol. 90, 1143–1147
Williamson, R.E., Ashley, C.C. (1982) Fre Ca2+ and cytoplasmic streaming in the alga Chara. Nature 296, 647–651
Willison, J.H.M. (1976) Plasmodesmata: a freeze-fracture view. Can. J. Bot. 54, 2842–2847
Zhang, D.H., Callaham, D.A., Hepler, P.K. (1990) Regulation of anaphase chromosome motion in Tradescantia stamen hair cells by calcium and related signaling agents. J. Cell Biol., in press
Author information
Authors and Affiliations
Additional information
This work was supported by Professional Staff Congress-City University (PSC-CUNY) of New York grant No. 667180 and U.S. Department of Agriculture grant No. 87-CRCR-1-244.
Rights and permissions
About this article
Cite this article
Tucker, E.B. Calcium-loaded 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid blocks cell-to-cell diffusion of carboxyfluorescein in staminal hairs of Setcreasea purpurea . Planta 182, 34–38 (1990). https://doi.org/10.1007/BF00239980
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00239980