Summary
Somatic polyploidy of species-specific and tissue-specific degrees occurs in almost all plant species studied so far, but nearly nothing is known about the control mechanisms switching the mitotic cycle to an endoreduplication cycle. In order to search for a possible role of the cdc2 kinase, cell suspension cultures of the Runner bean, Phaseolus coccineus (Leguminosae) were treated with K-252a, an inhibitor of protein kinase activity. The treatment resulted in continuous cell cycles without mitosis, and hence induced polyploidy levels up to 2048C. It is, therefore, suggested that phosphorylation of a protein kinase, probably of the cell cycle-important p34cdc2 type, is involved in the control of endoreduplication.
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Cavallini A, Cremonini R, Cionini G, Cionini PG (1988) Genome 30: 671–676
D'Amato F (1984) In: Novák FJ, Havel L, Dolezel J (eds) Proceedings of the International Symposium on Plant Tissue and Cell Culture, Czechoslovak Academy of Sciences, Praha, pp 295–303
Dutta A, Stillman B (1992) EMBO J 11: 2189–2199
Francis D (1992) New Phytol 122: 1–20
Gadbois DM, Hamaguchi JR, Swank RA, Bradbury EM (1992) Biochem Biophys Res Comm 184: 80–85
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158
Greber UF, Gerace L (1992) J Cell Biol 116: 15–30
Hamaguchi JR, Tobey RA, Pines J, Crissman HA, Hunter T, Bradbury EM (1992) J Cell Biol 117: 1041–1053
Hashimoto J, Hirabayashi T, Hayano Y, Hata S, Ohashi Y, Suzuka I, Utsugi T, Toh-E, A, Kikuchi Y (1992) Mol Gen Genet 233: 10–16
Hunt T (1992) Trends Biochem Sci 17: 1
Inzé D, Ferreira P, Hemerly A, Van Montagu M (1992) Biochem Soc Transact 20: 80–84
Matsuoka Y, Nishizawa K, Yano T, Shibata M, Ando S, Takahashi T, Inagaki M (1992) EMBO J 11: 2895–2902
Nagl W (1971) Z Naturforsch 26B: 1390–1391
Nagl W (1974) Z Pflanzenphysiol 73: 1–44
Nagl W (1978) Polyploidy and Polyteny in Differentiation and Evolution. North-Holland, Amsterdam
Nurse P (1990) Nature 344: 503–507
Nurse P (1991) Phil Trans R Soc London 332B: 271–276
Nye SH, Squinto SP, Glass DJ, Stitt TN, Hantzopoulos P, Macchi, MJ, Lindsay NS, Ip NY, Yancopoulos GC (1992) Molec Biol Cell 3: 677–686
Pelech SL, Sanghera JS (1992) Trends Biochem Sci 17: 233–238
Pelech SL, Sanghera JS, Daya-Makin M (1990) Biochem Cell Biol 68: 1297–1330
Planas-Silva MD, Means AR (1992) EMBO J 11: 507–517
Renshaw MW, Kipreos ET, Albrecht MR, Wang JYJ (1992) EMBO J 11: 3941–3951
Rosenblatt J, Gu Y, Morgan DO (1992) Proc Natl Acad Sci USA 89: 2824–2828
Rüegg UT, Burgess GM (1989) Trends Pharmacol Sci 10: 218–220
Smythe C, Newport JW (1992) Cell 68: 787–797
Usui T, Yoshida M, Abe K, Osada H, Isono K, Beppu T (1991) J Cell Biol 115: 1275–1282
Yamaguchi A, Yamashita M, Yoshikuni M, Hotta Y, Nurse P, Nagahama Y (1991) Devel Growth Diff 33: 625–632
Zucker RM, Adams DJ, Bair KW, Elstein KH (1991) Biochem Pharmacol 42: 2199–2208
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Nagl, W. Induction of high polyploidy in Phaseolus cell cultures by the protein kinase inhibitor, K-252a. Plant Cell Reports 12, 170–174 (1993). https://doi.org/10.1007/BF00239100
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DOI: https://doi.org/10.1007/BF00239100