Summary
The fine structure of ventromedial hypothalamic nucleus was studied in the rat with the electron microscope under circumstances of elevated brain monoamine level following treatment with a monoamine oxidase inhibitor Nialamid. The number of granular vesicles (size in diameter 450–1100 Å) in synaptic terminals increased after Nialamid treatment significantly, while their size did not change; the number of agranular vesicles remained unchanged. The time courses of the increase of granular vesicles and elevation of brain noradrenaline content were approximately parallel. It is inferred that the granular vesicles of size 450 to 1100 Å may possibly be the storage sites of noradrenaline.
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References
Aghajanian, G.K., and F.E. Bloom: Electron-microscopic autoradiography of rat hypothalamus after intraventricular H3-norepinephrine. Science 153, 308–310 (1966).
Bak, I.J.: Electron microscopic observations in the Substantia nigra of mouse during reserpine administration. Experientia (Basel) 21, 1–6 (1965).
Bak, I.J.: The ultrastructure of the Substantia nigra and caudate nucleus of the mouse and the cellular localization of catecholamines. Exp. Brain Res. 3, 40–57 (1967).
Bargmann, W., E. Lindner u. K.H. Andres: Über Synapsen an endokrinen Epithelzellen und die Definition sekretorischer Neurone. Z. Zellforsch. 77, 282–298 (1967).
Bloom, F.E., and R.J. Barrnett: Fine structural localization of noradrenaline in vesicles of autonomic nerve endings. Nature (Lond.) 210, 599–601 (1966).
Dahlström, A., and K. Fuxe: Evidence for the existence of monoamine-containing neurons in the central nervous system. Acta physiol. scand. 62, suppl. 232, 4–55 (1964).
De Robertis, E., A. Pellegrino de Iraldi, G. Rodriguez de Lores Arnaiz and L.M. Zieher: Synaptic vesicles from the rat hypothalamus. Isolation and norepinephrine content. Life. Sci. 4, 193–201 (1965).
Drujan, B.D., T.L. Sourkes, D.S. Layne and G.F. Murphy: The differential determination of catecholamines in urina. Canad. J. Biochem. 37, 1153–1159 (1959).
Fuxe, K., T. Hökfelt, O. Nilsson and S. Reinius: A fluorescence and electron microscopic study on central monoamine nerve cells. Anat. Rec. 155, 33–40 (1966).
Hashimoto, Y., S. Ishii, Y. Ohi, N. Shimizu and R. Imaizumi: Effect of dopa on the norepinephrine and dopamine contents and on the granulated vesicles of the hypothalamus of reserpinized rabbits. Jap. J. Pharmacol. 15, 395–400 (1965).
Hökfelt, T.: Electron microscopic studies on brain slices from regions rich in catecholamine nerve terminals. Acta physiol. scand. 69, 119–120 (1967a).
Hökfelt, T.: On the ultrastructural localization of noradrenaline in the central nervous system of the rat. Z. Zellforsch. 70, 110–117 (1967b).
Ishii, S., N. Shimizu, M. Matsuoka and R. Imaizumi: Correlation between catecholamine content and numbers of granulated vesicles in rabbit hypothalamus. Biochem. Pharmacol. 14, 183–184 (1965).
Matsui, I.: Effect of reserpine on the distribution of granulated vesicles in the mouse median eminence. Neuroendocrinology 2, 99–106 (1967).
Matsuoka, M., S. Ishii, N. Shimizu and R. Imaizumi: Effect of Win 18501–2 on the content of catecholamines and the number of the catecholamine-containing granules in the rabbit hypothalamus. Experientia (Basel) 21, 121–125 (1965).
Pellegrino de Iraldi, A., H. Farini Duggan and E. de Robertis: Adrenergic synaptic vesicles in the anterior hypothalamus of the rat. Anat. Rec. 145, 521–531 (1963).
Pellegrino de Iraldi, A., and E. de Robertis: Action of reserpine, iproniazid and pyrogallol on nerve endings of the pineal gland. Int. J. Neuropharmac. 2, 231–239 (1963).
Potter, L.T.: Storage of norepinephrine in sympathetic nerves. Pharmacol. Rev. 18, 439–451 (1966).
Reynolds, E.S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell. Biol. 17, 208–212 (1963).
Rinne, U.K., and A.U. Arstila: Electron microscopic evidence on the significance of the granular and vesicular inclusions of the neurosecretory nerve endings in the median eminance of the rat. Med. Pharmacol. exp. 15, 357–369 (1966).
Spector, S., K. Melmon and S. Sjoerdsma: Evidence for rapid turnover of norepinephrine in rat heart and brain. Proc. Soc. exp. Biol. (N.Y.) 111, 79–81 (1962).
Wolfe, D.E., J. Axelrod, L.T. Potter and K.C. Richardson: Localizing tritiated norepinephrine in sympathetic axons by electron microscope autoradiography. Science 138, 440–442 (1962).
Wood, J.G., and R.J. Barrnett: Histochemical demonstration of norepinephrine at a fine structural level. J. Histochem. Cytochem. 12, 197–209 (1964).
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Pfeifer, A.K., Szabó, D., Palkovits, M. et al. Correlation between noradrenaline content of the brain and the number of granular vesicles in rat hypothalamus during nialamid administration. Exp Brain Res 5, 79–86 (1968). https://doi.org/10.1007/BF00238698
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DOI: https://doi.org/10.1007/BF00238698