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Pattern of crypt cell proliferation in the pre- and post-closure ileum of the neonatal rat: Effects of sympathectomy

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Summary

The present experiments were designed to investigate ileal crypt cell division before and after neonatal closure to macromolecular absorption by measurements of mitotic index, labelling index, colchicine-induced estimation of mitotic rate (mitoses/cell/hour) and crypt depth and villus height on postnatal days 15–23. In addition, the effects of the sympathetic nervous system on crypt cell proliferation were analyzed by guanethidine-induced sympathectomy. Guanethidine treatment resulted in at least a 70 % reduction in superior cervical and coeliac perikarya at 15 days after birth. All cell proliferative indices demonstrated a rather constant rate of cell division in the ileal epithelium between 15 and 17 days after birth with a sudden burst of mitotic activity on day 18. The mitotic rate of control rats increased from day 18 to 23 with 3-week-old rat demonstrating a faster rate of ileal crypt cell proliferation than adult rats. The ileal crypt depth more than doubled between 15 and 23 days while the height of the villus column increased slowly but steadily (36 %) during the period of this study. Cell division is inhibited by guanethidine-induced sympathectomy although there is still an acceleration of mitosis in the post-closure period. The relationship of sympathectomy and ileal crypt cell proliferation is discussed and compared to hormonal effects on closure and related developmental events.

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References

  • Bullough WS (1952) Stress and epidermal mitotic activity. I. The effects of the adrenal hormones. J Endocrinol 8:265–274

    Google Scholar 

  • Bullough WS, Laurence EB (1968) The role of glucocorticoid hormones in the control of epidermal mitosis. Cell Tissue Kinet 1:5–10

    Google Scholar 

  • Clarke RM, Hardy RN (1969a) The use of (125I) polyvinyl pyrrolidone K.60 in the quantitative assessment of the uptake of macromolecular substances by the intestine of the young rat. J Physiol 204:113–125

    Google Scholar 

  • Clarke RM, Hardy RN (1969b) An analysis of the mechanism of cessation of uptake of macromolecular substances by the intestine of the young rat (‘closure’). J Physiol 204:127–134

    Google Scholar 

  • Dailey JW, Westfall TC (1978) Effects of adrenalectomy and adrenal steroids on norephinephrine synthesis and monoamine oxidase activity. Europ J Pharmacol 48:383–391

    Google Scholar 

  • Dupont JR, Biggers DC, Sprinz H (1965) Intestinal renewal and immunosympathectomy. Arch Pathol Lab Med 80:357–362

    Google Scholar 

  • Furness JB, Costa M (1974) The adrenergic innervation of the gastrointestinal tract. Rev Physiol Biochem Exp Pharmacol 69:1–51

    Google Scholar 

  • Halliday R (1955) The absorption of antibodies from immune sera by the gut of the young rat. J Endocrinol 18:56–66

    Google Scholar 

  • Herbst JJ, Sunshine P (1969) Postnatal development of the small intestine of the rat. Changes in mucosal morphology at weaning. Pediat Res 3:27–33

    Google Scholar 

  • Johnson EM, Cantor E, Douglas JR (1975) Biochemical and functional evaluation of the sympathectomy produced by administration of guanethidine to newborn rats. J Pharmacol Exp Ther 193:503–512

    Google Scholar 

  • Klein RM (1979a) Alteration of neonatal rat parotid gland acinar cell proliferation by guanethidine-induced sympathectomy. Cell Tissue Kinet 12:411–423

    Google Scholar 

  • Klein RM (1979b) Analysis of intestinal cell proliferation after guanethidine-induced sympathectomy. II. Percentage labelled mitoses studies. Cell Tissue Kinet 12:649–657

    Google Scholar 

  • Klein RM, Torres J (1978) Analysis of intestinal cell proliferation after guanethidine-induced sympathetomy. I. Stathmokinetic, labelling index, mitotic index, and cellular migration studies. Cell Tissue Res 195:239–250

    Google Scholar 

  • Koldovsky O, Sunshine P, Kretchmer N (1966) Cellular migration of intestinal epithelia in suckling and weaned rats. Nature 212:1389–1390

    Google Scholar 

  • Kulkarni AS, Shideman FE (1966) Sensitivities of the brains of infant and adult rats to the catecholamine-depleting actions of reserpine and tetrabenazine. J Pharmacol Exp Ther 153:428–433

    Google Scholar 

  • Lachat JJ, Goncalves RP (1978) Influence of autonomic denervation upon kinetics of the ileal epithelium of the rat. Cell Tissue Res 192:285–297

    Google Scholar 

  • Leblond CP, Stevens CE (1948) The constant renewal of the intestinal epithelium in the albino rat. Anat Rec 100:357–378

    Google Scholar 

  • Musso F, Lachat JJ, Cruz AR, Goncalves RP (1975) Effect of denervation on the mitotic index of the intestinal epithelium of the rat. Cell Tissue Res 163:395–402

    Google Scholar 

  • O'Connor T (1966) Cell dynamics in the intestine of the mouse from late fetal life to maturity. Am J Anat 118:525–536

    Google Scholar 

  • Patel AJ, Bailey R, Balazs R (1979) Effect of reserpine on cell proliferation and energy stores in the developing rat brain. Neuroscience 4:139–143

    Google Scholar 

  • Silva DG, Farrell KE, Smith GC (1968) Ultrastructural and histochemical studies on the innervation of the mucous membrane of the mouse colon. Anat Rec 162:157–176

    Google Scholar 

  • Tutton PJM (1973a) Proliferation of epithelial cells in the jejunal crypts of adrenalectomized and adrenocortical hormone treated rats. Virch Arch Abt B Zellpathol 13:227–232

    Google Scholar 

  • Tutton PJM (1973b) Stress induced inhibition of jejunal crypt cell proliferation. Virch Arch Abt B Zellpathol 15:23–34

    Google Scholar 

  • Tutton PJM (1975) Neural stimulation of mitotic activity in the crypts of Lieberkühn in rat jejunum. Cell Tissue Kinet 8:259–266

    Google Scholar 

  • Tutton PJM, Barkla DH (1976) A comparison of cell proliferation in normal and neoplastic intestinal epithelia following either biogenic amine depletion or monoamine oxidase inhibition. Virch Arch B Cell Pathol 21:161–168

    Google Scholar 

  • Tutton PJM, Helme RD (1974) The influence of adrenoreceptor activity on crypt cell proliferation in the rat jejunum. Cell Tissue Kinet 7:125–136

    Google Scholar 

  • Yeh KY (1977) Cell kinetics in the small intestine of suckling rats. I. Influence of hypophysectomy. Anat Rec 188:69–76

    Google Scholar 

  • Yeh KY, Moog F (1977) Influence of the thyroid and adrenal glands on the growth of the intestine of the suckling rat, and on the development of intestinal alkaline phosphatase and disaccharidase activities. J Exp Zool 200:337–348

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, University of Kansas Medical Center, College of Health Sciences and Hospital, Kansas City, Kansas, USA

    Robert M. Klein & James C. McKenzie

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  1. Robert M. Klein
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  2. James C. McKenzie
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Additional information

Supported by N.I.H. grant DE 04557

The authors wish to acknowledge Charles A. Brownley of CIBA-Geigy Summit, New Jersey, for the gift of guanethidine-sulfate

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Klein, R.M., McKenzie, J.C. Pattern of crypt cell proliferation in the pre- and post-closure ileum of the neonatal rat: Effects of sympathectomy. Cell Tissue Res. 206, 387–394 (1980). https://doi.org/10.1007/BF00237968

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  • DOI: https://doi.org/10.1007/BF00237968

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