Summary
The effect of ancymidol concentration on the development of haploid asparagus embryos was determined. Liquid cultures from anther-derived calli were grown for three weeks in MS medium plus 1.0 mg l−1 2,4-D, 0.1 mg l−1 NAA, 0.2 mg l−1 kinetin, 800 mg l−1 glutamine, 500 mg l−1 casein hydrolysate, 2% sucrose and 0.0–1.0 mg l−1 ancymidol. Cell clumps (224–500 μm) were plated on solid embryo maturation medium (MS medium plus 3% sucrose, 0.1 mg l−1 NAA, 0.5 mg l−1 kinetin and 0.0–1.0 mg l−1 ancymidol) and grown for eight weeks. Ancymidol enhanced embryo maturation and germination and was more critical in the solid than liquid medium. Total embryo number did not vary among most treatments. The best response was observed when ancymidol concentrations were 0.1 and 0.5 mg l−1 in the liquid and solid media, respectively; two-thirds of the embryos produced were bipolar and 35% of bipolar embryos germinated. Seven to 82% of plants recovered from different ancymidol treatments were haploid; the others were diploid, triploid or chimeric for ploidy level.
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Abbreviations
- NAA:
-
naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- MS:
-
Murashige and Skoog (1962)
References
Adler PR, Dufault RJ, Water L (1985) HortScience 20:196–198
Chin CK (1982) HortScience 17:590–591
Chin CK, Khunachak A (1984) Pl Physiol Suppl 75:100
Desjardins Y, Tiessen H, Harney PM (1987) HortScience 22:131–133
Feng XR, Wolyn DJ (1991) Pl Cell Rep 10:574–578
Grant WF (1982) In: Klekorski EJ (ed) Environmental Mutagenesis, Carcinogenesis and Plant Biology Vol II, Praeger, New York, pp 10–11
Hsu JY, Yen CC, Yang WC, Lin WC, Tsay HS (1990) Acta Hort 271:135–143
Levi A, Sink KC (1990) J Pl Physiol 137:184–189
Levi A, Sink KC (1991) Pl Cell Rep 10:71–75
Levi A, Sink KC (1992) Pl Cell Tissue Organ Culture 31:115–122
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Reuther G, (1977) Acta Hort 78:217–224
Saito T, Nishizawa S, Nishimura S (1991) Pl Cell Rep 10:230–234
Steward FC, Mapes MO (1971) Bot Gaz 133:70–79
Wilmar C, Hellendoorn M (1968) Nature 217:369–370
Zhan XC (1983) Acta Phytophysiol Sinica 9:317–325
Zhu Z (1982) Plant Chromosome Techniques, Academic Press, Beijing, China
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Communicated by E. D. Earle
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Feng, X.R., Wolyn, D.J. Development of haploid asparagus embryos from liquid cultures of anther-derived calli is enhanced by ancymidol. Plant Cell Reports 12, 281–285 (1993). https://doi.org/10.1007/BF00237136
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DOI: https://doi.org/10.1007/BF00237136