Abstract
Peanut (Arachis hypogea L.) peroxidase gene expression was analyzed by measuring the accumulation of trancripts in cultured cells and various plant parts (leaf, stem, root) and upon their treatment with ethylene or wounding, respectively. Two transcripts (prxPNC1 and prxPNC2) corresponding to two peroxidase genes are expressed at higher levels in cultured cells as compared to various plant organs. Analysis of total poly(A)+ RNA with an oligonucleotide probe corresponding to a highly conserved region of peroxidase genes showed the expression of three peroxidase related sequences (1,000, 1,400 or 2,600 bp) in stem or leaf but barely detectable in roots. The prxPNC2 transcript transiently expressed at high levels in response to ethylene treatment of cells or wounding of leaves. This suggests that the corresponding gene(s) are expressed in response to stress.
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Buffard D, Breda C, van Huystee RB, Asemota O, Pierre M, Dang Ha DB, Esnault R (1990) Proc. Natl. Acad. Sci. USA 87: 8874–8878
Chibbar RN, nvan Huystee RB (1983) Z. Pflanzenphysiol. 109: 191–196
Chibbar RN, Cella R, Albani D, van Huystee RB (1984) J. Exptl. Bot. 35: 1846–1852
Fujiyama K, Takemura H, Shibayama S, Kobayashi K, Choi JK, Shinmyo A, Takano M, Yamada Y, Okada H (1988) Eur. J. Biochem. 173: 681–687
Greppin H, Penel C, Gaspar T (1986). Molecular and Physiolo-gical Aspects of Plant Peroxidases, Univ. of Geneva, Switzer-land
Hall TC, Ma Y, Buchbinder BU, Pyne JW, Sun SM, Bliss FA (1978) Proc. Natl. Acad. Sci. USA 75: 3196–3200
Inoue M, van Huystee RB (1984) Can. J. Bot. 62: 1890–1895
Lagrimini LM, Burkhart W, Moyer M, Rothstein S (1987) Proc. Natl. Acad. Sci. USA, 84: 7542–7546
Lagrimini LM, Rothstein S (1987) Plant Physiol 84: 438–442
Mader M, Walter C (1986) Planta 119: 273–277
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Mohan R, Kollatukudy PE (1990) Plant Physiol. 921: 276–280
Morgens PH, Callahan AM, Dunn LJ, Abeles FB (1990) Plant Mol. Biol. 14: 715–725
Osborne D (1977) Sci. Prog. Oxf. 64: 53–65
Rebmann G, Hertig C, Bull J, Mauch F, Dudler R (1991) Plant Mol. Biol, 16: 329–331
Roberts E, Kutchan T, Kollatukudy PE (1988) Plant Mol. Biol. 11: 15–26
Shetty K, Bothra D, Crawford DL, Korus RA (1990) Appl. Bio-chem. Biotech. 24/25: 213–221
Stephan D, van Huystee RB (1981) Z. Pflanzenphysiol. 101: 313–321
Templeton MD, Lamb CJ (1988) Plant Cell Environ. 11: 395–401
Tessier L, Esnault R (1980) Mol. Cell Biochem. 29: 173–181
van Huystee RB (1987) Ann. Rev. Plant Physiol. 38: 205–219
van Huystee RB, Chibbar RN (1987) Isozymes: Current Topics in Biological and Medical Research 13: 155–179
Zheng X, van Huystee RB (1992) Plant Sc. 81: 47–56
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Communicated by F. Constabel
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Breda, C., Buffard, D., van Huystee, R.B. et al. Differential expression of two peanut peroxidase cDNA clones in peanut plants and cells in suspension culture in response to stress. Plant Cell Reports 12, 268–272 (1993). https://doi.org/10.1007/BF00237133
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DOI: https://doi.org/10.1007/BF00237133