Summary
In nembutal anesthetized adult cats, intracellular stimulation of single abducens motoneurones was used to elicit glycogen depletion of their muscle units. Stimulation by short trains (13 pulses at 40 Hz) delivered once a second, was applied for 20 to 110 min. The activation of the motor unit was monitored by intracellular recording of motoneurone action potentials and by EMG. After the end of stimulation, the muscle was excised and frozen to be cut in serial sections that were processed for demonstration of either glycogen, ATPases or SDH. In two experiments, a motor unit could be histochemically identified because 10–15 fibres showed zones of complete glycogen depletion measuring about 5 mm in length. All the depleted fibres had the same histochemical profile: ATPases reactions gave dark staining with alkaline preincubation and light staining with acid preincubation whereas SDH activity was low. In other experiments, prolonged stimulation produced either no depletion at all or very limited zones of partial depletion in a few muscle fibres.
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References
Asmussen G, Kiessling A, Wohlrab F (1971) Histochemical characteristics of muscle fibre types in the mammalian extraocular muscles. Acta Anat 79: 526–545
Barker D, Emonet-Denand F, Harker DW, Jami L, Laporte Y (1976) Distribution of fusimotor axons to intrafusal muscle fibres in cat tenuissimus spindles as determined by the glycogen-depletion method. J Physiol 261: 49–69
Barmack NH, Bell CC, Rence BG (1971) Tension and rate of tension development during isometric responses of extraocular muscle. J Neurophysiol 34: 1072–1079
Batini C, Buisseret-Delmas C, Kado RT (1979) On the fibres of the III, IV and VI cranial nerves of the cat. Arch Ital Biol 117: 111–122
Bowden REM (1960) The innervation of skeletal muscle. Brit Med J I: 671–674
Burke RE (1981) Motor units: anatomy, physiology and functional organization. In: The nervous system. Motor control. Am Physiol Soc, Bethesda Md (Handbook of physiology, Section 1, Vol II, Part 1, pp 345–422)
Burke RE, Levine DN, Tsairis P, Zajac FE (1973) Physiological types and histochemical profiles in motor units of the cat gastrocnemius. J Physiol 234: 723–748
Cooper S, Eccles JC (1930) The isometric responses of mammalian muscles. J Physiol 69: 377–385
Decorte L, Emonet-Denand F, Harker DW, Jami L, Laporte Y (1984) Glycogen depletion elicited in tenuissimus intrafusal muscle fibres by stimulation of static gamma-axons in the cat. J Physiol 346: 341–352
Destombes J, Gogan P, Rouviere A (1979) The fine structure of neurones and cellular relationships in the abducens nucleus in the cat. Exp Brain Res 35: 249–267
Dubowitz V, Brooke MH (1973) Muscle biopsy: a modern approach. WB Saunders Company Ltd, London, Philadelphia, Toronto
Duke-Elder S (1961) System of ophtalmology, Vol II. DV Mosby, Saint-Louis
Durston JHJ (1974) Histochemistry of primate extraocular muscles and the changes of denervation. Br J Ophtalmol 58: 193–216
Edström L, Kugelberg E (1968) Histochemical composition, distribution of fibres and fatigability of single motor units. Anterior tibial muscle of the rat. J Neurol Neurosurg Psychiat 31: 424–433
Fuchs AF, Binder MD (1983) Fatigue resistance of human extraocular muscles. J Neurophysiol 49: 28–34
Goldberg SJ, Lennerstrand G, Hull CD (1976) Motor unit responses in the lateral rectus muscle of the cat: intracellular current injection of abducens nucleus neurons. Acta Physiol Scand 96: 58–63
Goldberg SJ, Clamann HP, McClung JR (1981) Relations between motoneuron position and lateral rectus motor unit contraction speed: an intracellular study in the cat abducens nucleus. Neurosci Lett 23: 49–54
Grant K, Guéritaud JP, Horcholle-Bossavit G, Tyc-Dumont S (1979) Anatomical and electrophysiological identification of motoneurons supplying the cat retractor bulbi muscle. Exp Brain Res 34: 541–550
Grantyn R, Grantyn A (1978) Morphological and electrophysiological properties of cat abducens motoneurones. Exp Brain Res 31: 249–274
Guéritaud JP, Horcholle-Bossavit G, Jami L, Thiesson D, TycDumont S (1984a) Histochemical observations on an extraocular muscle of the cat: the rectus lateralis. Neurosci Lett Suppl 18: S229
Guéritaud JP, Horcholle-Bossavit G, Jami L, Thiesson D, TycDumont S (1984b) Glycogen depletion in motor units of the cat rectus lateralis muscle. Neurosci Lett Suppl 18: S 228
Henneman E, Somjen G, Carpenter DO (1965) Functional significance of cell size and spinal motoneurones. J Neurophysiol 28: 560–580
Highstein SM, Karabelas A, Baker R, McCrea RA (1982) Comparison of the morphology of physiologically identified abducens motor and internuclear neurons in the cat: a light microscopic study employing the intracellular injection of horseradish peroxidase. J Comp Neurol 208: 369–381
Kugelberg E (1973) Properties of the rat hindlimb motor units. In: Desmedt JE (ed) New developments in EMG and clinical neurophysiology, Vol 1. Karger, Basel, pp 2–13
Kugelberg E, Edström L (1968) Differential histochemical effects of muscle contractions on phosphorylase and glycogen in various types of fibres: relation to fatigue. J Neurol Neurosurg Psychiat 31: 415–423
Kugelberg E, Lindergren B (1979) Transmission and contraction fatigue of rat motor units in relation to succinate dehydrogenase activity in motor unit fibres. J Physiol 288: 285–300
Lennerstrand G (1974) Electrical activity and isometric tension in motor units of the cat's inferior oblique muscle. Acta Physiol Scand 91: 458–475
Lennerstrand G (1975) Motor units in eye muscles. In: Lennerstrand G, Bach-y-Rita P (eds) Basic mechanisms of ocular motility and their clinical implications. Pergamon Press, Oxford, pp 119–143
McDonagh JC, Binder MD, Reinking RM, Stuart DG (1980) Tetrapartite classification of motor units of cat tibialis posterior. J Neurophysiol 44: 696–712
Mayr R, Gottschall J, Gruber H, Neuhuber W (1975) Internal structure of cat extraocular muscle. Anat Embryol 148: 25–34
Nachlas MM, Tsou KC, De Sousa E, Cheng CS, Seligman AM (1957) Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J Histochem Cytochem 5: 420–436
Ringel SP, Engel WK, Bender AN, Peters ND, Yee RD (1978a) Histochemical and actylcholine receptor distribution in normal and denervated monkey extraocular muscles. Neurology 28: 55–63
Ringel SP, Wilson B, Barden MT, Kaiser KK (1978b) Histochemistry of human extraocular muscle. Arch Ophtalmol (Copenh) 96: 1067–1072
Spencer RF, Sterling P (1977) An electron microscope study of motoneurons and interneurons in the cat abducens nucleus identified by retrograde intra-axonal transport of horseradish peroxidase. J Comp Neurol 176: 65–86
Torre M (1953) Nombre et dimension des unités motrices dans les muscles extrinsèques de l'oeil et, en général, dans les muscles reliés à des organes des sens. Schweiz Arch Neurol Psychiat 72: 362–376
Wooten GF, Reis DJ (1972) Blood flow in extraocular muscle of cat. Arch Neurol 26: 350–352
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Supported by CNRS ATP no. 126 and by Fondation pour la Recherche Médicale
with the technical assistance of N. Seyfritz, INSERM
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Guéritaud, J.P., Horcholle-Bossavit, G., Jami, L. et al. Resistance to glycogen depletion of motor units in the cat rectus lateralis muscle. Exp Brain Res 60, 542–550 (1985). https://doi.org/10.1007/BF00236940
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DOI: https://doi.org/10.1007/BF00236940