Summary
Scale formation in Cyprinodon variegatus was found to be initiated at about 26 to 30 days after hatching. Ultrastructural investigation revealed that within 4 to 6 h in the first-formed scales the marginal cells begin to flatten and differentiate into osteogenic cells, which later change to osteoblasts and fibroblasts. These cells are separated from the surrounding epithelial cells by a basal lamina. The osteoid is formed by the marginal and osteogenic cells; the osseous layer by the osteoblasts; and the fibrillary plate by the fibroblasts.
The osteoid is formed within 2 to 3 h after the initiation of the scale, and within 20 to 24 h the osseous layer is formed. Hydroxyapatite crystals are deposited in the matrix of the osseous layer without apparent association with collagen fibers. No matrix vesicles or dense bodies are evident at the sites of calcification. The fibrillary plate arises 18 to 20 h after the initiation of the scale. It is also partially calcified, but not before the third week of scale formation. The crystals develop almost exclusively between the collagen fibers at the extreme edge of the calcifying front, but solid calcification of the fibers results with further growth of the crystals. The fibroblasts appear to participate in calcification of the fibrillary plate.
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References
Brown GA, Wellings SR (1969) Collagen formation and calcification in teleost scales. Z Zellforsch 93:571–582
Cooke PH (1967) Fine structure of the fibrillary plate in the central head scale of the striped killifish, Fundulus majalis. Trans Am Microsc Soc 86:273–279
DeLamater ED, Courtenay WR Jr (1974) Fish scales as seen by scanning electron microscopy. Florida Sci 37:141–149
Fagade SO (1973) Age determination in Tilapia melanotheran (Ruppell) in the Lagos Lagoon, Lagos, Nigeria. In: Bagenal TB (ed) The Aging of Fish. Unwin Brothers, Surrey, England, pp 71–77
Fouda MM (1979) Studies on scale structure in the common goby Pomatoschistus microps Krøyer. J Fish Biol 15:173–183
Glimcher MJ, Krane SM (1968) The organization and structure of bone, and the mechanism of calcification. In: Gould BS (ed) Treatise on collagen, Vol 2, Pt. B Academic Press, New York, pp 67–251
Goodrich ES (1907) On the scales of fish, living and extinct, and their importance in classification. Proc Zool Soc Lond 2:751–774
Kobayashi S, Yamada J, Maekawa K, Ouchi K (1972) Calcification and nucleation in fish-scales. Biomineralization 6:84–90
Lanzing WJR, Higgenbotham DR (1967) Scanning microscopy of surface structure of Tilapia mossambica (Peters) scales. Fish Biol 6:307–310
Maekawa K, Yamada J (1972) Morphological identification and characterization of cells involved in the growth of the goldfish scales. Jp J Ichthyol 19:1–10
Olson OP (1976) Histochemical and developmental studies of the scale of the sheepshead minnow Cyprinodon variegatus. Master of Science, University of South Carolina, Columbia, South Carolina, pp 1–151
Olson OP, Watabe N (1980) Histochemical studies of the scales of the sheepshead minnow Cyprinodon variegatus. (In preparation)
Onozato H, Watabe N (1979) Studies on fish scale formation and resorption. III. Fine structure and calcification of the fibrillary plates of the scales in Carassius auratus (Cypriniformes:Cyprinidae). Cell Tissue Res. 201:409–422
Onozato H, Watabe N (1980) Scanning electron microscopic study on calcification of the fibrillary plate of the scales in Carassius auratus. (In preparation)
Onozato H, Yamada J, Watabe N (1980) Denticle structure of teleost fish scales. (In preparation)
Oosten J Van (1957) The skin and scales. In: Brown ME (ed) The Physiology of Fishes, Academic Press, New York, Vol. 1, pp 207–244
Schönbörner AA, Boivin G, Baud CA (1979) The mineralization processes in teleost fish scales. Cell Tissue Res. 202:203–212
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct. Res 26:31–43
Waterman RE (1970) Fine structure of scale development in the teleost, Brachydanio rerio. Anat Rec 168:361–380
Yamada J (1971) A fine structural aspect of the development of scales in the chum salmon fry. Bull Jp Soc Sci Fish 37:18–29
Yamada J, Watabe N (1979) Studies on fish scale formation and resorption. I. Fine structure and calcification of the scales in Fundulus heteroclitus (Atheriniformes:Cyprinodontidae). J Morphol 159:49–66
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Contribution No. 332, Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina, Columbia, South Carolina, 29208, USA
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Olson, O.P., Watabe, N. Studies on formation and resorption of fish scales. Cell Tissue Res. 211, 303–316 (1980). https://doi.org/10.1007/BF00236451
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DOI: https://doi.org/10.1007/BF00236451