Summary
Horseradish peroxidase (HRP) has been used as a protein tracer in order to visualize the ultrastructural sites of the orthograde transport of protein macromolecules in the hypothalamo-neurohypophysial tract of the rat. After a local injection of HRP within the supraoptic nucleus, the reaction product was observed: (1) mainly in tubules of the smooth endoplasmic reticulum in the more proximal part of the axons, and (2) in granules and microvesicles of the axon terminals. Observations on thick sections clearly showed the existence of a relationship between the smooth endoplasmic reticulum containing HRP and the labeled granules or microvesicles. These data are in good agreement with previous findings showing the existence of direct continuity between tubules of the smooth endoplasmic reticulum and a fraction of the neurosecretory granules and microvesicles. This evidence further reinforces the hypothesis that the latter organelles may possibly originate locally in the axons from the tubules of the smooth endoplasmic reticulum which may therefore be proposed as a possible vehicle for a non-granular intra-axonal transport of neurosecretory material in neurosecretory neurons.
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Alonso, G., Assenmacher, L: The smooth endoplasmic reticulum in neurosecretory axons of the rat neurohypophysis. Biol. Cell. 32, 203–206 (1978)
Alonso, G. Boudier, J.C., Tasso, F.: Localisation dans la neurohypophyse du rat normal et déshydraté de protéines néoformées à partir de H3-Leucine, par radioautographie à haute résolution. C.R. Acad. Sci. 279, 1471–1474 (1974)
Alonso, G., Rambourg, A., Assenmacher, I.: Transport axonal du matériel neurosécrétoire dans le système hypothalamo-posthypophysaire: rôle du réticulum endoplasmique lisse. J. Physiol. (Paris) 72, p6B (1976)
Alonso, G., Rambourg, A., Assenmacher, I.: Relationships between the smooth endoplasmic reticulum and the axonal organelles within neurosecretory neurons. Proc. Internat. Union Physiol. Sci. 13, p. 18 (1977)
Bargmann, W.: Über die neurosekretorische Verknüpfung von Hypothalamus und Neurohypophyse. Z. Zellforsch. 34, 610–634 (1949)
Dellmann, H.D., Rodríguez, E.M.: Comparative study of the ultrastructure and hormonal content of the proximal and distal stumps of the transected neurosecretory hypothalamo-hypophysial system. Experientia 26, 414–415 (1970)
Droz, B., Koenig, H.L., Rambourg, A.: Transport intracytoplasmique de macromolécules et réticulum endoplasmique lisse: cas du flux axonal rapide. J. Microsc. (Paris) 20, 45a (1974)
Gerschenfeld, H.M., Tramezzani, J.H., De Robertis, E.: Ultrastructure and function in neurohypophysis of the toad. Endocrinol. 66, 741–763 (1960)
Graham, R.C., Karnovsky, H.J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubule of the mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966)
Heller, H., Lederis, K.: Density gradient centrifugation of hormone containing granules from rabbit neurohypophysis. J. Physiol. (Lond.) 158, 27–28 (1961)
Holder, F.C.: Arguments expérimentaux en faveur de la localisation intragranulaire des hormones hypothalamo-neurohypophysaires chez Anguilla anguilla L. Z. Zellforsch. 140, 315–332 (1973)
Jones, C.W., Pickering, B.T.: Intra-axonal transport and turnover of neurohypophysial hormones in the rat. J. Physiol. (Lond.) 227, 533–564 (1972)
Kalimo, H.: Ultrastructural studies on the hypothalamic neurosecretory neurons of the rat. II. The hypothalamo-neurohypophysial system in rats with hereditary hypothalamic diabetes insipidus. Z. Zellforsch. 134, 205–225 (1972)
Kalimo, H.: Ultrastructural studies on the hypothalamic neurosecretory neurons of the rat. III. Paraventricular and supraoptic neurons during lactation and dehydration. Cell Tissue Res. 163, 151–168 (1975)
La Vail, J.H., Winston, K.R., Tish, A.: A method based on retrograde intra-axonal transport of protein for identification of cell bodies of origin of axons terminating within the CNS. Brain Res. 58, 470–477 (1973)
Norström, A., Sjöstrand, J.: Axonal transport of proteins in the hypothalamo-neurohypophysial system of the rat. J. Neurochem. 18, 28–39 (1971a)
Norström, A., Sjöstrand, J.: Transport and turnover of neurohypophysial proteins of the rat. J. Neurochem. 18, 2007–2016 (1971b)
Palay, S.L.: An electron microscope study of the neurohypophysis in normal hydrated and dehydrated rats. Anat. Rec. 121, 348 (1955)
Pickering, B.T., Jones, C.W.: The biosynthesis and intraneuronal transport of neurohypophysial proteins: preliminary studies in the rat. Mem. Soc. Endocr. 19, 327–351 (1971)
Price, P., Fisher, A.W.: Electron microscopic study of retrograde axonal transport of horseradish peroxidase in the supraoptico-hypophysial tract in the rat. J. Anat. 125, 137–147 (1978)
Reperant, J.: The orthograde transport of horseradish peroxidase in the visual system. Brain Res. 85, 307–312 (1975)
Sotelo, C., Riche, D.: The smooth endoplasmic reticulum and the retrograde and fast orthograde transport of horseradish peroxidase in the nigro-striatal-nigral loop. Anat. Embryol. 146, 209–218 (1974)
Zambrano, D., De Robertis, E.: The secretory cycle of supraoptic neurons in the rat. A structural functional correlation. Z. Zellforsch. 73, 414–431 (1964)
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Alonso, G., Assenmacher, I. Electron microscopic study of the orthograde axonal transport of horseradish peroxidase in the supraoptico-neurohypophysial tract of the rat. Cell Tissue Res. 194, 525–531 (1978). https://doi.org/10.1007/BF00236171
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DOI: https://doi.org/10.1007/BF00236171