Summary
Distribution of succinic dehydrogenase (SDH) activity in various parts of neurons, perikarya, dendrites, axons and axon terminals was studied at the electron microscope (EM) level by means of a copper-ferrocyanide method recently developed by xxKerpel-Fromus and xxHajos (1967). Mitochondria of the perikarya, both in the CNS and in ganglia, show a marked SDH activity, which is still strong in proximal parts of the dendrites, but which greatly decreases in more distal parts of the dendrites. SDH activity becomes hardly detectable in the axons and appears consequently lacking in the synaptic axon terminals. It is assumed, however, that this is due to a level of activity that is below the sensitivity of the method employed. Cytochrome oxidase activity has been shown in earlier studies by the same authors not to be different in mitochondria of perikarya and axon terminals. The possible significance of this striking difference in somal and synaptic mitochondria of neurons is briefly discussed.
Similar content being viewed by others
References
xxChappell, J.B.: Integrated oxidations in isolated mitochondria. In: Biological Structure and Function. New York and London: Academic Press 1961.
—, and K.N. xxHaarhoff: The penetration of the mitochondrial membranes by anions and cations. In: Biochemistry of Mitochondria, pp. 75–91. New York and London: Academic Press 1967.
xxDroz, B., and C.P. xxLeblond: Axonal migration of proteins in the central nervous system and peripheral nerves as shown by radioautography. J. comp. Neurol. 121, 325–346 (1963).
xxEccles, J.C., M. xxIto and J. xxSzentágothai: The Cerebellum as a Neuronal Machine. Berlin-Heidelberg-New York: Springer 1967.
xxFox, C.A., D.E. xxHillmann, K.A. xxSiegesmund and C.R. xxDutta: The primate cerebellar cortex. A Golgi and electron microscope study. In: Progress in Brain Research, vol. 25. Amsterdam-London-New York: Elsevier 1967.
xxGerebtzoff, M.A.: Histoenzymologie de l'étranglement de Ran vier dans la fibre myélinisée périphérique normale et en dégénérescence. Ann. Histochem. 9, 209–215 (1964).
—, et J. xxBrotchi: Implications fonctionelles de recherches histoenzymologiques sur les fibres nerveuses. J. Physiol. (Paris) 57, 614–615 (1965).
—: Localisations et activités d'enzymes oxydo-réducteurs dans le nerf et le ganglion rachidien. Ann. Histochim. 11, 63–70 (1966).
xxHámori, J., and J. xxSzentágothai: The Purkinje-cell baskets. Ultrastructure of an inhibitory synapse. Acta biol. Acad. Sci. hung. 15, 465–479 (1965).
xxKása, P., and B. xxCsillik: Personal communication (1965).
xxKekpel-Fronius, S., and F. xxHajós: A method for the electron microscopic demonstration of cytochrome oxidase in fresh and formalin-perfixed tissues. Histochemie 10, 216–223 (1967).
—: The use of ferrocyanide for the light and electron microscopic demonstration of succinic dehydrogenase activity. Histochemie 14, 343–351 (1968).
xxNandy, K., and G.H. xxBourne: A histochemical study of the localization of succinic dehydrogenase, cytochrome oxidase and diphosphopyridine nucleotide-diaphorase in the synaptic regions of the spinal cord in the rat. J. Histochem. Cytochem. 12, 188–196 (1964a).
—: A histochemical study of the localization of the oxidative enzymes in the neurons of the spinal cord in rats. J. Anat. (Lond.) 98, 647–653 (1964h).
xxOgawa, K., and R.J. xxBarrnett: Electron cytochemical studies of succinic dehydrogenase and dihydronicotinamide-adenine dinucleotide diaphorese activities. J. Ultrastruct. Res. 12, 388–508 (1965).
—: The site of ferricyanide reduction by reductases within mitochondria as studied by electron microscopy. J. Histochem. Cytochem. 16, 49–57 (1968)
xxReynolds, E.S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell. Biol. 17, 208–212 (1963).
xxRoberts, N.R., R.R. xxCoelho, O.H. xxLowry and E.I. xxCrawford: Enzyme activities of giant squid axoplasm and axon sheath. J. Neurochem. 3, 109–115 (1958).
xxSeligman, A.M., H. xxUeno, J. xxMorizono, J.L. xxWasserkrug, L. xxKatzoff an J.S. xxHanker: Electron microscopic demonstration of dehydrogenase activity with a new osmiophilic tetrazolium salt (TC-NBT). J. Histochem. Cytochem. 15, 1–13 (1967).
xxShanta, T.R., K. xxIjima and G.H. xxBourne: Histochemical studies on the cerebellum of squirrel monkey. Acta Histochem. 27, 129–162 (1967).
xxShimizu, N., and T. xxAbe: Histochemical studies of the brain with reference to glucose metabolism. In: Progress in Brain Research, vol. 21/A, pp. 197–216. Amsterdam-London-New York: Elsevier 1966.
xxSinger, T.P., and E.B. xxKearney: Determination of succinic dehydrogenase activity. In: Methods of Biochemical Analysis, vol. 4, pp. 307–333. New York and London: Interscience 1957.
xxSze-Chuh-Cheng, R. xxNahamura and H. xxWaelsch: Relative contribution of carbon dioxide fixation and acetyl-CoA pathways in two nervous tissues. Nature (Lond.) 216, 928–929 (1967).
xxSzentágothai, J.: Zum elementaren Bau der interneuronalen Synapse. Acta anat. (Basel) 30, 827–841 (1957).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hajós, F., Kerpel-Fronius, S. Electron histochemical observation of succinic dehydrogenase activity in various parts of neurons. Exp Brain Res 8, 66–78 (1969). https://doi.org/10.1007/BF00234926
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00234926