Summary
Mature rats raised on a vitamin D deficient diet were injected with tritium labeled 1,25 dihydroxyvitamin D3. Concentration of radioactivity, which is prevented by pretreatment with unlabeled 1,25 dihydroxyvitamin D3, is found in nuclei of cells that are centrally located in pancreatic islets. The central location of these cells and supportive evidence from the literature suggest that they are B-cells, and that 1,25 dihydroxyvitamin D3 has a direct and genomic action on B-cell functions including insulin secretion.
This is a preview of subscription content, log in via an institution to check access.
References
Berney PW (1952) Osteoporosis and diabetes mellitus. J Iowa Med Soc 42:10–12
Boquist L, Hagström S, Strindlund L (1977) Effect of 1,25 dihydroxycholecalciferol administration on blood glucose and pancreatic islet morphology in mice. Acta Pathol Microbiol Scand Sect A 85:489–500
Christakos S, Norman AW (1979) Immunoreactive calcium binding protein and receptor/binding protein for 1,25 (OH)2-vitamin D3 in chick pancreas. Abstract 756 — Program and abstracts of the 61st annual meeting of the Endocrine Society
DeFronzo RA, Cooke CR, Andres R, Davis PJ (1975) The effect of insulin on the handling of sodium, potassium, calcium and phosphate in man. J Clin Invest 55:845–855
DeFronzo RA, Goldberg M, Agus ZS (1976) The effects of glucose and insulin on renal electrolyte transport. J Clin Invest 58:83–90
DeLuca HF (1978) The hormonal nature of vitamin D function. In: Dumont J, Nunez J (eds) Hormones and Cell Regulation, Vol 2, Elsevier/N Holland Biomed Press, pp 249–270
Dixit P, Stern AMK (1979) Effect of insulin on the incorporation of citrate and calcium into the bones of alloxan diabetic rats. Calcif Tissue Intl 27:227–232
Grodsky GM, Bennett LL (1966) Cation requirements for insulin secretion in the isolated perfused pancreas. Diabetes 15:910–913
Haussler MR, Pike JW, Chandler JS, Jones PG (1980) Molecular mechanism of action of 1,25 dihydroxy-vitamin D hormone. Program and abstracts VI International Congress Endocrinology
Heath III H, Lambert PW, Service FJ, Arnaud SB (1979) Calcium homeostasis in diabetes mellitus. J Clin Endocrinol Metabol 49:462–466
Hellman B (1975) The significance of calcium for glucose stimulation of insulin release. Endocrinology 97:392–398
Kim H, Kalkhoff RK, Costrini NV, Cerletty JM, Jacobson M (1971) Plasma insulin disturbances in primary hyperparathyroidism. J Clin Invest 50:2596–2605
Levin ME, Boisseau VC, Avioli LV (1976) Effects of diabetes mellitus on bone mass in juvenile and adult-onset diabetes. N Engl J Med 294:241–245
Littledike ET, Witzel DA, Whipp SC (1968) Insulin: Evidence for inhibition of release in spontaneous hypocalcemia. Proc Soc Exp Biol Med 129:135–139
McNair P, Madsbad S, Christiansen C, Christensen MS, Faber OK, Binder C, Transbol I (1979) Bone loss in diabetes: Effects of metabolic state. Diabetologica 17:283–286
Menczel J, Makin M, Robin G, Jaye I, Naor E (1972) Prevalence of diabetes mellitus in Jerusalem: Its association with presenile osteoporosis. Isr J Med Sci 8:918–919
Milner RDG, Hales CN (1967) The role of calcium and magnesium in insulin secretion from rabbit pancreas studied in vitro. Diabetologica 3:47–49
Morrissey RL, Bucci TJ, Empson RN Jr, Lufkin EG (1975) Calcium-binding protein: Its Cellular localization in jejunum, kidney and pancreas. Proc Soc Exp Biol Med 149:56–60
Narbaitz R, Stumpf WE, Sar M (1980) The role of autoradiographic and immunocytochemical techniques in the clarification of sites of metabolism and action of vitamin D. J Histochem Cytochem (in press)
Ramamurthy NS, Zebrowski EJ, Baker C, Golub LM (1973) Alloxan diabetes and reduced bone density in rat mandible. Res Commun Chem Pathol Pharmacol 5:614
Raskin P, Stevenson MRM, Barilla DE, Pak CYC (1978) The hypercalciuria of diabetes mellitus: Its amelioration with insulin. Clin Endocrinol 9:283–288
Sar M, Stumpf WE, DeLuca HF (1980) Thyrotropes in the pituitary are target cells for 1,25 (OH)2 vitamin D3. Cell Tissue Res 209:161–166
Schneider LE, Schedl HP (1972) Diabetes and intestinal calcium absorption in the rat. Am J Physiol 223:1319–1323
Schneider LE, Omdahl J, Schedl HP (1976) Effects of vitamin D and its metabolites on calcium transport in the diabetic rat. Endocrinology 99:793–799
Schneider LW, Nowosielski LM, Schedl HP (1977a) Insulin treatment of diabetic rats: Effect on duodenal calcium absorption. Endocrinology 100:67–73
Schneider LE, Schedl HP, McCain T, Haussler MR (1977b) Experimental diabetes reduces circulating 1,25-dihydroxyvitamin D in the rat. Science 196:1452–1454
Stumpf WE, Sar M (1975) Autoradiographic techniques for localizing steroid hormones. In: O'Malley BW, Hardman JG (eds) Methods in Enzymology, Vol XXXVI, Hormone Action, Part A. Steroid Hormones. Academic Press, NY, pp 135–156
Stumpf WE, Sar M, Reid FA, Tanaka Y, DeLuca HF (1979) Target cells for 1,25-dihydroxyvitamin D3 in intestinal tract, stomach, kidney, skin, pituitary and parathyroid. Science 206:1188–1190
Stumpf WE, Sar M, Narbaitz R, Reid FA, DeLuca HF, Tanaka Y (1980) Cellular and subcellular localization of 1,25 (OH)2 vitamin D3 in rat kidney. Comparison with that of parathyroid hormone and estradiol. PNAS 77:1149–1153
Suda T, DeLuca HF, Tanaka Y (1970) Biological activity of 25-hydroxyergocalciferol in rats. J Nutrition 100:1049–1052
Sulimovici S, Roginsky MS (1979) Effect of diabetes in rats on the metabolism of cholecalciferol and 25-hydroxycholecalciferol. In: Norman AW, Schaeffer K, Herrath DV, Grigoleit HG, Coburn JW, DeLuca HF, Mawer EB, Suda T (eds) Vitamin D, Basic Research, and its Clinical Application. Walter de Gruyter, Berlin-NY, pp 979–981
Wu K, Schubeck KE, Frost HM, Villanueva A (1970) Haversian bone formation rates determined by a new method in a mastodon and in human diabetes mellitus and osteoporosis. Calcif Tissue Res 6:204–219
Yasuda K, Hurukawa Y, Okuyama M, Kikuchi M, Yoshinaga K (1975) Glucose tolerance and insulin secretion in patients with parathyroid disorders. N Engl J Med 292:501–504
Author information
Authors and Affiliations
Additional information
Supported by US Public Health Service Grants NS 09914 and AM 14881
Rights and permissions
About this article
Cite this article
Clark, S.A., Stumpf, W.E., Sar, M. et al. Target cells for 1,25 dihydroxyvitamin D3 in the pancreas. Cell Tissue Res. 209, 515–520 (1980). https://doi.org/10.1007/BF00234764
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00234764